An improved flask system for the growth of extremely oxygen-sensitive bacteria in liquid culture is described. The improvement described utilizes an all-glass, neoprene-stoppered flask designed for growth of 50- to 1,000-ml cultures of bacteria with continuous gassing.

Chinese hamster ovary (CHO) cells is one of the most widely used production host for the commercial production of biopharmaceuticals product. They have been extensively studied and developed, and today provide a stable platform for producing monoclonal antibodies and recombinant proteins. This study focused on the production of recombinant protein in suspension culture of CHO cells in spinner f...

BACKGROUND Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate. Determination and optimization of these parameters through experiments are labor-intensive and time-consuming. Computational Fluid Dynamics (CFD) provides the ability to p...

PT XYZ adalah perusahaan di Indonesia yang bergerak dalam bidang retail memiliki lebih dari 14.300 toko serta 32 cabang atau warehouse. Masalah muncul ketika telah menentukan harga suatu barang dan harus disebarkan ke setiap toko. Harga akan selalu berubah karena adanya promo diskon masa periode nya sendiri, sehingga diperlukan penyebaran secara real-time. Selain itu, dengan jutaan transaksi ti...

A robust oxygen-flask combustion system was developed using light beam ignition. An appropriate amount of environmental sample was wrapped with a small piece of filter paper and held in a basket made of platinum-zirconium spiral. After the sample was inserted into an oxygen-filled flask containing an acid mixture, the light beam from an overhead projector lamp was focused to ignite the sample. ...