detection of bluetongue virus in aborted lamb fetuses in chaharmahal va bakhtiari and isfahan provinces, by rt-pcr method background: bluetongue or muzzle sore disease is an infectious disease which is mainly transmitted by insects. it can cause erosions in mouth, nasal mucosa and limbs and also abortion in ruminants. the aim of this study was genomic detection of bluetongue virus in sheep abor...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

background and aims: a multiplex transcription-polymerase chain reaction (m-pcr) was developed for direct detection and discrimination between canarypox virus (cpv) and other avian poxvirus (apv). materials and methods: three compatible primer sets were designed for m-pcr amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved apv genes. results: results showed th...

widespread occurrence of h9n2 low pathogenic avian influenza (lpai) viruses in many asian countriesduring the past decade has resulted in the need for evaluation of the pathogenesis of h9n2 virus infection. in this study, tissue tropism and dissemination of a/chicken/iran/772/1998(h9n2) virus throughout the body of broiler chickens were investigated. the clinical signs, gross lesions and antibo...

Background: Approximately 600,000 deaths occur every year as a result of the acute and chronic consequences of hepatitis B virus infection. Ten different hepatitis B virus genotypes have been identified with distinct geographical distributions. Different clinical outcomes, including the rate of mutations, development of hepatocellular carcinoma, chronicity, response to treatment, transplantatio...

herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. during latent infection, virus genome exhibit limited transcription, with the hsv lats consistently detected in latency infected ganaglia. following ocular infection viral latency develops in the trigeminal ganglia. in this study pcr has been used for detection of hsv-1 nucli...

Plum pox or sharka, a viral disease caused by Plum pox virus (PPV), severely affects the production of Prunus species in Europe. The first evidence of sharka was reported in Iran in 2000 . Due to the economic impact of this disease on crop production, recent advances in the term of  biology, epidemiology, and disease management are provided in this paper to assure awareness among growers and pr...

In this study a label-free electrochemical Immunosensor for ultrasensitive detection of Hepatitis C virus core antigen in serum samples was fabricated by using a simple approach. In this method a low-cost and sensitive immunosensor was fabricated based on a boehmite nanoparticles (BNPs) modified glassy carbon. The BNPs provide a specific platform with increased surface area which is capable of ...

Newcastle disease virus (NDV), avian influenza virus (AIV), and infectious bronchitis virus (IBV) are the most prevalent viral pathogens in the Iranian poultry industry. This study aimed to reveal the presence of these viruses in the backyard chickens in Ahvaz, located in the Southwest of Iran. A total of 100 chickens with respiratory signs and mortality were examined by taking the blood sample...