Pneumoviruses are responsible for significant respiratory disease in their hosts and represent a major problemfor human and animal health. Pneumoviruses are members of the family Paramyxoviridae, subfamilyPneumovirinae and the virus particles consist of a negative-sense, nonsegmented RNA genome within a helical nucleocapsid structure enveloped in a lipid membrane derived from the ho...

abstract during the winter and spring of 2007 and 2008, 450 samples (leaves of carnation plants) showing symptoms of yellowing, mottling, leaf malformation, and stunting were collected from greenhouses in mashhad and chenaran regions. the samples were tested by das-elisa for the presence of carmv. total rna was extracted by rnxtm (-plus) solution from positive infected samples confirmed in das-...

Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 ...

Background and Aims: Crimean-Congo hemorrhagic fever (CCHF) is a zoonosis caused by a Nairovirus of the family Bunyaviridae. Infection is transmitted to humans mostly by Hyalomma ticks. This study was conducted to determine the rate of CCHFV infection in ticks in Qom province of Iran. Materials and Methods: In this study, Reverse transcription – polymerase chain reaction (RT-PCR) was used...

Extracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component EVs. However, the proportion of small sequencing (smRNA-seq) reads EVs mapped to human genome much lower than that cells, suggesting existence many nonhuman sequences there no systematic study on EV fragments unmapped genome. Herein, using smRNA-seq data, landscape exogen...

Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...

the varicella-zoster virus (vzv) genome contains both cis-acting and trans-acting elements, which are important in viral dna replication. the cis-acting elements consist of two copies of oris, and the trans-acting elements are those genes whose products are required for virus dna replication. it has been shown that each of the seven genes required for ori-dependent dna synthesis of herpes simpl...

In this study, we report the complete genome sequence of swinepox virus from a clinical sample naturally occurring infection in India. The sequencing was done on Nanopore MinION sequencer Oxford Technologies. Two new annotations were added to genome. Three genes found have frameshifts, which might be importance relation infection. When compared only other reported whole virus, obtained an isola...