Gene silencing is an important tool in the study of gene function. Virus-induced gene silencing (VIGS) and hairpin RNA interference (hpRNAi), both of which rely on small interfering RNAs, together with artificial microRNAs (amiRNA), are amongst the most popular methods for reduction of gene activity in plants. However, all three approaches have limitations. Here, we introduce miRNA-induced gene...

results in this study, seven sirna molecules were rationally designed against the nucleoprotein gene and validated using various computational methods for silencing different strains of rsv. additionally, three effective sirna molecules targeting the overlapping region of m2/l mrna were designed. conclusions this approach provides insight and a validated strategy for chemical synthesis of an an...

The phytoene desaturase (PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting PDS expression leads to appearance of mottled, chlorosis, albino leaves. In this study, CDS sequence EuPDS (Eucommia ulmoides Phytoene Desaturase) was first cloned and then silenced Nicotiana benthamiana. Result showed level leaves higher than that roots stems. N. benthamiana l...

conclusions by employment of gene optimization strategies, use of viral-based vectors and suppression of plant-derived gene silencing effect, efficient transient expression of hcvcp in tobacco with proper antigenic properties could be possible. background hepatitis c virus (hcv) is major cause of liver cirrhosis in humans. hcv capsid (core) protein (hcvcp) is a highly demanded antigen for vario...

Abstract Background Virus-induced gene silencing (VIGS) is a useful tool for functional characterizations of plant genes. However, the penetrance VIGS varies depending on genes to be silenced, and has evaluated by examining transcript levels target Results In this report, we report development novel vector that permits preliminary assessment penetrance. This new based an attenuated variant Turn...

Eggplant (Solanum melongena L.), a widely cultivated vegetable of the Solanaceae family, faces significant challenges in growth and yield due to soil salinization. This study aimed investigate functional role transcription factor SmMYB39 salt stress tolerance eggplant. investigation was conducted through utilization bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-...