In vitro fertilization [5] (IVF) is an assisted reproductive technology [6] (ART) initially introduced by Patrick Steptoe [7] and Robert Edwards [8] in the 1970s to treat female infertility [9] caused by damaged or blocked fallopian tubes [10]. This major breakthrough in embryo research has provided large numbers of women the possibility of becoming pregnant, and subsequent advances have dramat...

Volume 2 • Issue 3 • 1000108 J Fert In Vitro ISSN: 2165-7491 JFIV, an open access journal Abbreviations: NE: Nuclear Envelope, NPC: Nuclear Pore Complex, NRN: Nuclear Routing Network, Np: Nucleoplasmin, H: histone, tRNA: Transfer Ribonucleic Acid, mRNA: Messenger Ribonucleic Acid, NLS: Nuclear Localization Signal, NES: Nuclear Export Signal, gDNA: Genomic DNA, ISH: In Situ Hybridization, scFv: ...

Human in vitro fertilization has become a widely accepted method of treatment for infertile couples. The list of indications for this technique is rapidly growing and includes oligospermia, endometriosis, and unexplained infertility. The techniques of follicular recruitment, ovum maturation, and follicular aspiration, as well as laboratory culture techniques, are described. The various factors ...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

In vitro growth culture systems for oocytes are being developed in several mammalian species. In these growth culture systems, in vitro grown oocytes usually have lower blastocyst formation than in vivo grown oocytes after in vitro fertilization. Furthermore, there have been a few reports that investigated the fertilization ability of in vitro grown oocytes in large animals. The purpose of this...

BACKGROUND As discontinuation in in vitro fertilization (IVF) programs has been associated with a poor prognosis, one hypothesis is that some couple-specific predictive factors in IVF may be shared with opposite effect by both success (i.e. live birth) and treatment discontinuation processes. Our objective was to perform a joint analysis of these two processes to examine the hypothesis of a lin...

Although conventional cryopreservation is a proven method for long-term, safe storage of genetic material, protocols used by the zebrafish community are not standardized and yield inconsistent results, thereby putting the security of many genotypes in individual laboratories and stock centers at risk. An important challenge for a successful zebrafish sperm cryopreservation program is the large ...