Proteins known to function during cell-cell communication and exocytosis in neurons and other secretory cells have recently been reported in human sperm. Synapsins are a group of proteins that have been very well characterized in neurons, but little is known about synapsin function in other cell types. Based upon previous findings and the known function of synapsin, we tested the hypothesis tha...

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

We have recently presented evidence that the structural integrity of the mouse sperm nuclear matrix may be necessary for the proper unpackaging of sperm DNA for participation in embryogenesis. It is likely that the sperm nuclear matrix contributes to the organisation of the sperm DNA and its disturbance can seriously damage the paternal genome or its expression. In this work, we confirm our pre...

Background: Fertilization and fecundation obviously are the phenomenon in which several factors are involved. Sperm membranous proteins are a series of these important elements. CD9, an inner acrosomal membrane protein, is one of the tetraspanin members that involved in sperm-egg fusion. CD52, is a GPI anchor protein which is expressed in the epididymal cells and during passage of sperms throug...

We compare a set of convolutional neural network (CNN) architectures for the task of segmenting and detecting human sperm cells in an image taken from a semen sample. In contrast to previous work, samples are not stained or washed to allow for full sperm quality analysis, making analysis harder due to clutter. Our results indicate that training on full images is superior to training on patches ...

Garrido N, Meseguer M, Bellver J, Remohi J, Simon C and Pellicer A (2004) Report of the results of a 2 year programme of sperm wash and ICSI treatment for human immunodeficiency virus and hepatitis C virus serodiscordant couples. Hum Reprod 19,2581–2586. Mencaglia L, Falcone P, Lentini GM, Consigli S, Pisoni M, Lofiego V, Guidetti R, Piomboni P and De Leo V (2005) ICSI for treatment of human im...

BACKGROUND The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the sperm cells' progressive motility concentration (PMC) in a l...

Background: Low uptake of exogenous DNA by sperm and reduced number of fertilized oocyte by transfected sperm are the major obstacles for progression of sperm mediated gene transfer. Therefore, the modification of sperm mediated gene transfer procedure needs to be required. The purpose of this study was to evaluate the efficiency of FuGene 6 compare to lipofection in transfection medium for int...

OBJECTIVE Sperm DNA undergoes apoptotic fragmentation when exposed to HPV DNA. Details of the specific gene regions targeted by HPV in sperm are lacking. The objective of this study was to determine the integrity of exons 5 and 8 of the p53 gene in sperm exposed to HPV DNA. METHODS Washed sperm were exposed to either HLA-DQA1 (control) or HPV type 6b/11, 16, 18, 31, or 33 DNA fragments for 24...