The aim of the present study was to investigate the effect of osteopontin (OPN) on adhesion and migration in human primary renal cortical epithelial cells during wound healing and Transwell assays. MTT assay was used to examine the cell viability and western blot analysis was used to examine the expression of cytoskeletal proteins and cell adhesion molecules. The results showed that overexpress...

Confocal microscopy analysis of fluorescence and morphology is becoming the standard tool in cell biology and molecular imaging. Accurate quantification algorithms are required to enhance the understanding of different biological phenomena. We present a novel approach based on image-segmentation of multi-cellular regions in bright field images demonstrating enhanced quantitative analyses and be...

Denervated skin could result in impaired healing of wounds, such as decubitus ulcers and diabetic foot ulcers. Other studies indicated that cutaneous fiber density is reduced after inner nerve transection and that neuropeptide level depletes after denervation, leading to reduced cell proliferation around the wound and thus wound healing problems. Recent studies have revealed that skin-derived p...

ETHNOPHARMACOLOGICAL RELEVANCE Traditional healers in Nigeria employ a range of plant preparations as wound healing agents. Despite the use of local plants in wound healing, there is only scant literature on the wound healing properties of these plants to support the continued therapeutic application of these herbal remedies. AIM OF THE STUDY To document plants commonly used to treat wounds i...

BACKGROUND Activin B has been reported to promote the proliferation and migration of keratinocytes in vitro via the RhoA-JNK signaling pathway, whereas its in vivo role and mechanism in wound healing process has not yet been elucidated. PRINCIPAL FINDINGS In this study, we explored the potential mechanism by which activin B induces epithelial wound healing in mice. Recombinant lentiviral plas...

AIM OF THE STUDY The present study was undertaken to evaluate the wound healing effects of the four chief saponins of Astragalus species [cycloastragenol (CA), astragaloside IV (AG), cyclocephaloside I (CCI) and cyclocanthoside E (CCE)]. MATERIAL AND METHODS Effects of cell viability and proliferation of the isolated compounds were evaluated by the MTT assay on human keratinocyte. The wound h...

Wound healing assays are important for molecular biologists to understand the
 mechanisms of cell migration. For the analysis wound assays, accurate
 segmentation front is a necessity. Manual annotation is
 inconvenient since it time-consuming and annotator-dependent. Thus automated, fast,
 robust solutions required. There several image processing techniques
 proposed f...

Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells (ECs) are useful substrates for wound healing. The present study investigates feasibility covalent conjugation taurine (Tau) on a gelatin-based hydrogel. This hydrogel is expected to maintain positive charged growth factors such as basic fibroblast factor (bFGF) vascular (VEGFs) near ECs within microenvironm...