in recent years, symptoms of a viral disease including streak mosaic, leaf blade malformation, and thickness (scrawniness) of raceme have been observed in the vast areas of grain fields in golestan province. in order to identify the causes of this disease, detection of wheat streak mosaic virus (wsmv) in cultivated hosts including wheat, dominant weeds of fields involving barnyardgrass (echinoc...

Mutations within the HC-Pro coding region of Wheat streak mosaic virus (WSMV) were introduced by misincorporation during PCR and evaluated for phenotype within the context of an infectious clone. Nine synonymous substitutions and 15 of 25 non-synonymous substitutions had no phenotypic effect. Four non-synonymous substitutions, including one that reverted consistently to wild type, resulted in a...

23 24 Viral coat proteins function in virion assembly and virus biology in a tightly coordinated manner 25 with a role for virtually every amino acid. In this study, we demonstrated that the coat protein 26 (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus; family Potyviridae) is 27 unusually tolerant of extensive deletions with continued virion assembly and/or systemic 28 infection....

Seifers, D. L., and Martin, T. J. 1988. Correlation of low level wheat streak mosaic virus resistance in Triumph 64 wheat with low virus titer. Phytopathology 78:703-707. Wheat streak mosaic virus (WSMV) reduced the yield of Triumph 64 immunosorbent assay (ELISA). As incubation temperature increased from (cultivar having a low level of resistance to virus multiplication) by 18% 15 to 30 C, diff...

Abstract Wheat streak mosaic virus (WSMV) is a common wheat causing economic losses to production in the Great Plains of North America. Reproducible inoculation WSMV by mechanical methods essential evaluate resistance breeding lines and relies on successful infectivity particles. We used reverse transcription-quantitative PCR (RT-qPCR) for absolute quantification viral genome copy numbers both ...

Viral determinants and mechanisms involved in extension of host range of monocot-infecting viruses are poorly understood. Viral coat proteins (CP) serve many functions in almost every aspect of the virus life cycle. The role of the C-terminal region of Wheat streak mosaic virus (WSMV) CP in virus biology was examined by mutating six negatively charged aspartic acid residues at positions 216, 28...

Wheat streak mosaic tritimovirus (WSMV) seriously damages wheat worldwide. We report analyses of new complete ORF (CO) sequences from seven Australian isolates with 56 COs and 128 coat protein (CP) genes sequenced previously. Eleven CO three CP were recombinants so removed our analyses. Patristic distances maximum-likelihood phylogenies nonrecombinant (n-rec) their closely correlated (R = 0.994...

Triticum mosaic virus (TriMV) and High plains (HPV), identified recently, have been considered among the major viruses that affect wheat. Carried by same vector, wheat curl mite, both of these cause yellowing stunting plants which are very similar to many other attacking This makes it difficult detect in different lines, posing a problem yield. paper highlights addition two multiplex RT-PCR bas...