We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glyco...

The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...

The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...

Yeast cells are protected by a cell wall that plays an important role in the exchange of substances with the environment. The cell wall structure is dynamic and can adapt to different physiological states or environmental conditions. For the investigation of morphological changes, selective staining with fluorescent dyes is a valuable tool. Furthermore, cell wall staining is used to facilitate ...

The fungal cell wall viewed through the electron microscope appears transparent when fixed by the conventional osmium tetroxide method. However, ruthenium tetroxide post-fixing has revealed new details in the ultrastructure of Penicillium sp. hyphae and Saccharomyces cerevisiae yeast. Most significant was the demonstration of two or three opaque diverse electron dense layers on the cell wall of...

Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.

AFM has been used for a high-resolution imaging of the surface topography of dried yeast cells fixed on glass slide. Differences between yeast cells cultured in media with 0.2% and 2% glucose concentration are presented. Though we intend to examine yeast cells under native condition in aqueous media later, we used this simplest method of sample preparation in this preliminary study. This study ...