Resistance to β-lactam antibiotics mediated by metallo-β-lactamases (MBLs) is a growing problem. We describe the use of protein-observe 19 F-NMR (PrOF NMR) to study the dynamics of the São Paulo MBL (SPM-1) from β-lactam-resistant Pseudomonas aeruginosa. Cysteinyl variants on the α3 and L3 regions, which flank the di-ZnII active site, were selectively 19 F-labeled using 3-bromo-1,1,1-trifluoroa...

New molecular complexes of fullerenes C60 and C70 with tetraphenylporphyrins [M(tpp)] in which M-H2, MnII, CoII, CuII, ZnII and Fe(III)Cl, have been synthesised. Crystal structures of two C60 complexes with H2TPP, which differ only in the number of benzene solvated molecules, and C60 and C70 complexes with [Cu(tpp)] have been studied. The fullerene molecules form a honeycomb motif in H2TPP.2C60...

Many existing irrigation, industrial and chemical storage sites are currently introducing hazardous anions into groundwater, making the monitoring of such sites a high priority. Detecting and quantifying anions in water samples typically requires complex instrumentation, adding cost and delaying analysis. Here we address these challenges by development of an optical molecular method to detect a...