The authors apologise for this error. Figure 4. His-tag effect on uneven distribution and activity of chloramphenicol acetyltransferase (CAT), neopullulanase (BCN) and esterase 1767 (A) Distinct uneven distribuiton of proteins with N-terminal His-tag were observed by immunofluorescence staining using anti-His-tag antibody. As a negative conrol, unlocalized patterns of mBFP (without His-tag) usi...

Three evergreen (Laurus nobilis, Viburnum tinus and Thuja plicata) and two autumnal abscission deciduous trees (Cydonia oblonga and Prunus domestica) have been investigated for the presence (zymogram and immunodetection) and functionality (post-illumination chlorophyll fluorescence) of the thylakoid Ndh complex. The presence of encoding ndh genes has also been investigated in T. plicata. Wester...

The effects of crude plasma and ethanol-extracted swamp eel at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality tilapia (Oreochromis mossambicus) surimi were investigated. parameters analyzed gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern by sodium dodecyl sulfate-polyacrylamide electrophore...

The Sunn pest, Eurygaster integriceps Puton (Heteroptera: Scutelleridae), is one of the most important pests of wheat and causes considerable damage to this valuable crop annually. Digestive proteinase activity of adult insects was investigated using general and specific substrates and inhibitors. Proteolytic activity was low when the common conventional substrates, azoalbumin, azocasein and he...

A, digestive gland; B, buccal cavity; C, mantle muscle; D, eye; E, brain. The lower margin of the photograph is the position reached by bromophenol blue. The L-LDH was specifically isolated by affinity chromatography before application to the tops of gels (see text). Electrophoresis was carried out in tubes of 7.5% (w/v) polyacrylamide at pH 8.3 according t o the method of Davis 1.51. LDH was v...