Respiratory chain analysis of high ethanol producing Zymomonas mobilis mutants. 1 2 Takeshi Hayashi* Tsuyoshi Kato, and Kensuke Furukawa 3 4 Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu 5 University, Beppu, Oita 874-8501, Japan 6 Food Science and Nutrition, Graduate School of Food Science and Nutrition, Beppu 7 University, Beppu, Oita 874-8501, Japan 8 9 *Co...

BACKGROUND Acetic acid is a predominant by-product of lignocellulosic biofuel process, which inhibits microbial biocatalysts. Development of bacterial strains that are tolerant to acetic acid is challenging due to poor understanding of the underlying molecular mechanisms. RESULTS In this study, we generated and characterized two acetic acid-tolerant strains of Zymomonas mobilis using N-methyl...

Mutants of Zymomonas mobilis were selected for decreased alcohol dehydrogenase activity by using consecutively higher concentrations of allyl alcohol. A mutant selected by using 100 mM allyl alcohol produced acetaldehyde at a level of 4.08 g/liter when the organism was grown in aerated batch cultures on a medium containing 4.0% (wt/wt) glucose. On the basis of the amount of glucose utilized, th...