We have evaluated the potential use of various polyamidoamine (PAMAM) dendrimer [dendrimer, generation (G) 2-4] conjugates with cyclodextrins (CyDs) as novel DNA and RNA carriers. Among the various dendrimer conjugates with CyDs, the dendrimer (G3) conjugate with α-CyD having an average degree of substitution (DS) of 2.4 [α-CDE (G3, DS2)] displayed remarkable properties as DNA, shRNA and siRNA ...

background: there is no convenient cheap pragmatic experimental model for nonalcoholic fatty liver disease (nafld)/nonalcoholic steatohepatitis (nash). we aimed to create a pragmatic model of nafld/nash. methods: sprague-dawley rats were fed a high-fat, high sugar homemade diet ad libitum for seven weeks. the high-fat, high sugar diet included 59% of energy derived from fat, 30% from carbohydra...

BACKGROUND Alpha-melanocyte-stimulating-hormone (α-MSH) has marked anti-inflammatory potential. Proinflammatory cytokines are critical mediators of the disturbed cartilage homeostasis in osteoarthritis, inhibiting anabolic activities and increasing catabolic activities in chondrocytes. Since human chondrocytes express α-MSH receptors, we evaluated the role of the peptide in modulating chondrocy...

Background: Pre-eclampsia is the most common critical condition during pregnancy. Plasma concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta (IL-1β) increase in pregnant women with pre-eclampsia, compared to normal pregnant women. Objective: To investigate the polymorphisms of IL-1β (C+3954T), TNF-α (G- 308A), and (G-238A) in preeclemptic women northeastern Iran. Me...

INTRODUCTION Cases with microcytosis not responding adequately to iron supplementation are diagnostic dilemma and have been reported to harbor alpha (α) thalassemia mutations. The aim of this study was to determine the common α globin gene deletions in cases with microcytic anemia. METHODS Fifty four patients selected (22 females and 32 males) had microcytic anemia (MCV < 80 fl, Hb <12gm/dl) ...

BACKGROUND Fabry disease (FD) is a rare disease due to an X-linked recessive inborn error of glycosphingolipid metabolism resulting from the mutations of the α-galactosidase A (α-gal A) gene. FD is rare in Chinese and the data on clinic and genetic features of FD is still limited. METHODS In this study, the α-gal A gene of a Chinese family diagnosed with FD was analyzed for mutations and the ...

Screening for producers of potent antimicrobial peptides, resulted in the isolation Bacillus cereus BGNM1 with strong activity against Listeria monocytogenes. Genome sequence analysis revealed that contains gene cluster associated production lantibiotic, thusin, previously identified B. thuringiensis. Purification confirmed strain BGMN1 produces thusin. Both thusin sensitive and resistant strai...

The high-voltage-activated calcium channel is a multimeric protein complex containing α(1), α(2)/δ, β, and γ subunits. The α(1) subunit is the ion conduction channel and contains the binding sites for calcium channel blockers and toxins. Three genes code for distinct L-type, dihydropyridine-sensitive α(1) subunits; one gene codes for the neuronal P-type (Purkinje) α(1) subunit; and one gene cod...

The aim of this study was to compare the effect five types PEGlated nanoliposomes (PNLs) on α-synuclein (α-syn) fibrillization, attenuation microglial activation, and silence SNCA gene, which encodes α-syn. To evaluate inhibition α-syn we used standard in vitro assay based Thioflavin T (ThT) fluorescence. Next, concentration TNF-a IL-6 quantified by ELISA BV2 microglia cells treated with 100 nM...