BACKGROUND The use of silica coated magnetic nanoparticles as contrast agents has resulted in the production of highly stable, non-toxic solutions that can be manipulated via an external magnetic field. As a result, the interaction of these nanocomposites with cells is of vital importance in understanding their behaviour and biocompatibility. Here we report the preparation, characterisation and...

The effects of the sulfhydryl reducing agents 2-mercaptoethanol and dithiothreitol on wortmannin-induced inhibition of phosphoinositide 3-kinase (PI3K) were studied in order to examine whether the sulfhydryl reducing agents directly affect the wortmannin inhibition of PI3K. These reducing agents are commonly used to stabilize enzyme structures by maintaining protein sulfhydryl groups in the red...

Abstract Understanding N uptake by plants, the cycle, and their relationship to soil heterogeneity has generated a great deal of interest in distribution amino-N compounds soil. Visualization spatial can provide insights into role labile plant-microbial mechanisms acquisition plant uptake, but until now, it remained technically challenging. Here, we describe novel technique visualize at root-so...

Alkaline proteases are the most important group of industrial enzymes that hydrolyse peptide bond proteins into small peptides. The demand alkaline protease predominantly from microbial origin has been recently increased and enhances research for with high stability at extreme conditions. Thus, this study is aimed to characterize bacterial isolate, Bacillus subtilis VBC7 screened dairy waste du...

Recent studies suggest that the H1 subunit of the carbohydrate recognition domain (H1CRD) of the asialoglycoprotein receptor is used as an entry site into hepatocytes by hepatitis A and B viruses and Marburg virus. Thus, molecules binding specifically to the CRD might exert inhibition towards these diseases by blocking the virus entry site. We report here the identification of the epitope struc...

2-Mercaptoethanol inhibits growth of Aspergillus parasiticus NRRL 3240 and aflatoxin formation by the fungus. When added to the resuspended medium, 2-mercaptoethanol inhibited [1-14C]acetate incorporation into both aflatoxins and neutral lipids, thereby showing that it acts at an early stage of aflatoxin biosynthesis. The inhibition is probably due to its chelating action on zinc, which is esse...

The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) widely used efficient method to obtain pure RNA most cells. However, it not with some like sperm because they are resistant chaotropic lysis solutions containing guanidinium thiocyanate such as Buffer RLT+ Trizol. Here, we show th...