The chitosan-induced coacervation of milk proteins was investigated using a proteomic approach. The addition of 0.8% chitosan to milk caused the milk proteins to coacervate after a 1 h incubation period. Approximately 86% of the milk proteins were present in the milk pellet fraction (MPF), and the protein concentration of the milk supernatant fraction (MSF) decreased from 29.4±0.2 to 4.2±0.6 mg...

Our objective was to perform a genome-wide association study for content in bovine milk of α(S1)-casein (α(S1)-CN), α(S2)-casein (α(S2)-CN), β-casein (β-CN), κ-casein (κ-CN), α-lactalbumin (α-LA), β-lactoglobulin (β-LG), casein index, protein percentage, and protein yield using a 50K single nucleotide polymorphism (SNP) chip. In total, 1,713 Dutch Holstein-Friesian cows were genotyped for 50,22...

The objective was to evaluate the effect of body condition score (BCS) at 30 days before calving (-30 days) induced by a differential nutritional management, parity and week of lactation (WOL) on milk yield and composition, and milk casein and fatty acid composition. Primiparous and multiparous Holstein cows with high BCS (PH, n = 13; MH, n = 9) and low BCS (PL, n = 9; ML = 8) under grazing con...

In the structural-based mutagenesis of Mucor pusillus pepsin (MPP), understanding how κ-casein interacts with MPP is a great challenge for us to explore. Chymosin-sensitive peptide is the key domain of κ-casein that regulates milk clotting through the specific proteolytic cleavage of its peptide bond (Phe¹⁰⁵-Met¹⁰⁶) by MPP to produce insoluble para-κ-casein. Here, we built the model of this lar...

The relationships between goat κ-casein (CSN3) variants and milk composition were studied in 1143 goats from 4 breeds (Camosciata, Frisa, Orobica, Verzasca). Individual milk samples were analysed by isoelectrofocusing. The effect of CSN3 type on protein and casein content was evident, B variant being associated to higher levels of both traits. Since the B variant is mainly present in local capr...

An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...