Clinical criteria, microsatellite analysis (MSA) and immunohistochemistry (IHC) are important diagnostic tools for identification of hereditary nonpolyposis colorectal cancer (HNPCC) patients who are likely to carry pathogenic germline mutations in mismatch repair genes. Based on MSA and IHC results and subsequent mutation analyses of 1,119 unrelated index patients meeting the Amsterdam II crit...

In order to determine whether lithocholic acid (LCA) contributes to the occurrence of total parenteral nutrition (TPN) associated intrahepatic cholestasis (IHC) in neonates, we investigated the serum bile acid fractions of neonates on TPN. Twenty-five surgical neonates, receiving TPN for more than 2 weeks were studied. TPN associated IHC was defined as serum defect bilirubin greater than 2.0 mg...

OBJECTIVE The relationship between inflammation, obesity, and adverse metabolic conditions is associated with adipose tissue macrophages (ATM). This study compared the measurements of human ATM using flow cytometry, immunohistochemistry (IHC), and real-time polymerase chain reaction (RT-PCR) of ATM markers. METHODS A new software program (AMCounter) was evaluated to help measure ATM using IHC...

BACKGROUND Based on the excellent results of the clinical trials with ALK-inhibitors, the importance of accurately identifying ALK positive lung cancer has never been greater. However, there are increasing number of recent publications addressing discordances between FISH and IHC. The controversy is further fuelled by the different regulatory approvals. This situation prompted us to investigate...

BACKGROUND High frequencies of the BRAF V600E mutation have been reported in pleomorphic xanthoastrocytoma (PXA). Recently, a BRAF V600E mutation-specific antibody has been developed and validated. We evaluated the immunohistochemical (IHC) detection of BRAF V600E mutation in PXA by comparing to gold standard molecular analysis and investigating the interobserver variability of the IHC scoring....

Overexpression of HER2 is one of the major causes of breast cancer, and therefore precise diagnosis of its protein expression level is important. However, current methods estimating the HER2-expression level are insufficient due to problem with the lack of quantification. This might result in a gap between diagnostics and therapeutics targeting HER2. Therefore, a new effective diagnostic method...

Although immunohistochemistry (IHC) is a popular imaging technique, the quantitative analysis of IHC images via computer-aided methods is an emerging field that is gaining more and more importance thanks to the new developments in digital high-throughput scanners. In this paper, we discuss the main steps of IHC and review the techniques for computer-aided chromogenic IHC analysis, including met...

By Dean Chang EDITORIAL ASSISTANT To attain the lofty status of All-American would require a combination o f talent, hard work and determination. Going into Ihc Nationals held at Emory University in Atlanta, none of Ihc five women nor their coach of Albany Slate's women's swim team expected thni much from themselves. The Nationals would be a solid learning experience for Ihc groups, and would i...

BACKGROUND Integrative health care (IHC) combines therapies and providers from complementary and conventional health care. Previous studies on IHC have shown power relations between providers but few studies have explored how the interaction develops over time. The objective of this study was to explore the development of IHC collaboration and interaction among participating providers during a ...

This paper aims at presenting a personal experience in teaching the HCI course at the Computer Science Department at the Federal University of Minas Gerais, in Brazil. The course syllabus, how theory and practice are integrated in the course, and the experience to integrate the course work and the Evaluation Competition category available at the Brazilian Symposium of Human Factors in Computing...