The role of RNA structure in virtually any biological process has become increasingly evident, especially the past decade. However, classical approaches to solving structure, such as crystallography or cryo-EM, have failed keep up with rapidly evolving field and need for high-throughput solutions. Mutational profiling sequencing using dimethyl sulfate (DMS) MaPseq is a sequencing-based approach...

adenylate kinases (adk) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. in this study, the cloning of a cdna encoding an adenylate kinase from the filariaonchocerca volvulus has been described. using pcr technique, a 281 bp cdna fragment encoding part ofan adenylate kinase was isolated from an o. volvulus cdna library. use of this fragment as a p...

Bacteriophages that are routinely used in cDNA libraries do not require any biological selection for forming plaques. Thus parental non-recombinant phages are always found in variable proportions together with recombinant ones in all cDNA libraries. The presence of non-recombinants in significant proportions dilutes the abundance of rare cDNA species and makes library screening difficult. If th...

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, ...

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transf...

Studying complex metazoan communities requires taxonomic expertise and laborious work if done using the traditional morphological approach. Nowadays, popular use of molecular25 based methods accompanied by massively parallel sequencing (MPS) provides rapid higher resolution diversity analyses. However, estimates derived from molecular-based approach can be biased co-detection environmental DNA ...

هدف: همان‌گونه که مشهود است، در سازمان‌های خدماتی به‌منظور برندسازی نیاز است ارزش‌های برندشان فعال و نهادینه شود؛ با این پیش‌فرض، پژوهش پیش‌رو هدف استخراج مدل فعال‌سازی برند داخلی از منظر فعالیت‌های مدیریت منابع انسانی تعریف اجرا شده است.طراحی/ روش‌شناسی/ رویکرد: روش مطالعۀ موردی رویکرد کیفی بانک ملت برای توسعۀ پیش گرفته شد. دو مرحلۀ اصلی طراحی گردید مصاحبه‌های عمیق فردی ۱۹ مدیر کارشناس مربوطه ...