Recent RNA-seq studies reveal that the transcriptomes in animals and plants are more complex than previously thought, leading to the inclusion of many more splice isoforms in annotated genomes. However, it is possible that a significant proportion of the transcripts are spurious isoforms that do not contribute to functional proteins. One of the current hypotheses is that commonly used mRNA extr...

يا هچروم يدنب هشوخ متيروگلا ياراد پ ا ار يددعتم ياهرتم هلمج زا ،نتشادرب هب طوبرم ياهرتماراپ نتشاذگ اه هداد ديد عاعش ، يم هك دشاب ريثات و دنراد متيروگلا ييارگمه و دركلمع رد يدايز اطخ و شيامزآ تروص هب لاومعم نييعت يم رگ د دن . شور هلاقم نيا رد ي رب ينتبم CLA-PSO 1 ي هتسسگ لدم كي هك PSO دشاب يم يارب قيبطت كيتاموتا پ ياهرتمارا يا هچروم يدنب هشوخ يم داهنشيپ ددرگ . روظنم هب گلا ساسا رب هك د...

Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a bottleneck to wider adoption. Here we report a novel method for the production of strand specific RNA-seq librarie...

Taxonomic characterization of active gastrointestinal microbiota is essential to detect shifts in microbial communities and functions under various conditions. This study aimed to identify and quantify potentially active rumen microbiota using total RNA sequencing and to compare the outcomes of this approach with the widely used targeted RNA/DNA amplicon sequencing technique. Total RNA isolated...

To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both RNA-Seq and microarray analyses were performed on RNA samples from a human T cell activation experiment. In contrast to other reports, our analyses focused on the difference, rather than similarity, between RNA-Seq and microarray technologies in transcriptome profiling. A comparison of data sets derived from...

كچ ي هد فده و هقباس : شبات ،هتشذگ ههد لوط رد ناديم زا يشان يريگ نتنآ زا لصاح يسيطانغمورتكلا ياه هدـنريگ ياه / ت هدنتسرف تسا هتفاي شيازفا هارمه نفل . هعلاطم نيا In vivo جاوما رثا يسررب روظنم هب 950 نـفلت متسيس زترهاگم هارمه GSM ينلاوط تيوقت رب تدم ) LTP ( هيحان Dentate gyrus تفرگ تروص . شور و داوم اه : 32 ر أ داژن زا ركذم يهاگشيامزآ گرزب شوم س Wistar دودح ينس اب 3 نزو و هام 15 ± 220 ـ گ ،مر...

The application of next-generation sequencing (NGS) to transcriptomics, commonly called RNA-seq, allows the nearly complete characterization of transcriptomic events occurring in a specific tissue. It has proven particularly useful in nonmodel species, which often lack the resources available for sequenced organisms. Mainly, RNA-seq does not require a reference genome to gain useful transcripto...

Use of second generation sequencing (SGS) technologies for transcriptional profiling (RNA-Seq) has revolutionized transcriptomics, enabling measurement of RNA abundances with unprecedented specificity and sensitivity and the discovery of novel RNA species. Preparation of RNA-Seq libraries requires conversion of the RNA starting material into cDNA flanked by platform-specific adaptor sequences. ...

RNA-seq has been widely adopted as a gene-expression measurement tool due to the detail, resolution, and sensitivity of transcript characterization that the technique provides. Here we present two transposon-based methods that efficiently construct high-quality RNA-seq libraries. We first describe a method that creates RNA-seq libraries for Illumina sequencing from double-stranded cDNA with onl...