نتایج جستجو برای: تکنیک lamp
تعداد نتایج: 37531 فیلتر نتایج به سال:
background and aims: considering difficulties in usual laboratory methods in detection of viral infections, improved dna-based diagnostic techniques are more reliable. loop mediated isothermal amplification method (lamp) is a nucleic acid amplification method that amplifies dna using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. in this study, the...
conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...
Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDN...
We introduce LAMP: the Linear Additive Markov Process. Transitions in LAMP may be influenced by states visited in the distant history of the process, but unlike higher-order Markov processes, LAMP retains an efficient parameterization. LAMP also allows the specific dependence on history to be learned efficiently from data. We characterize some theoretical properties of LAMP, including its stead...
∗ Corresponding author Abstract -This exploratory study examined the possibility of driving a fluorescent lamp at high frequency with no serially connected passive elements. The proposed driver is a full bridge stage controlled by the phase shifted PWM scheme. Current feedback was used to stabilize the average of the rectified lamp current to a set value. The experimental results of this resear...
BACKGROUND & AIMS The pathogenic mechanism of pancreatitis is poorly understood. Recent evidence implicates defective autophagy in pancreatitis responses; however, the pathways mediating impaired autophagy in pancreas remain largely unknown. Here, we investigate the role of lysosome associated membrane proteins (LAMPs) in pancreatitis. METHODS We analyzed changes in LAMPs in experimental mode...
A genetic vaccine for West Nile virus (WN) has been synthesized with the WN premembrane-envelope (WN preM-E) gene sequences encoded as a chimera with the transmembrane and carboxyl terminal domains of the lysosome-associated membrane protein (LAMP). The LAMP sequences are used to direct the antigen protein to the major histocompatibility class II (MHC II) vesicular compartment of transfected pr...
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