Both transcriptional activation and repression have essential functions in maintaining proper spatial and temporal control of gene expression. Although Wnt signalling is often associated with gene activation, we have identified several directly repressed targets of Wnt signalling in Drosophila. Here, we explore how individual Wnt target genes are specified for signal-induced activation or repre...

Thermal creep flow (TCF) is a flow of gas driven by a temperature gradient along a solid boundary. Here, TCF is demonstrated experimentally in a dusty plasma. Stripes on a glass box are heated by laser beam absorption, leading to both TCF and a thermophoretic force. The design of the experiment allows isolating the effect of TCF. A stirring motion of the dust particle suspension is observed. By...

Tool condition forecasting (TCF) is a key technology for continuous drilling of CFRP/Ti stacks, as the tool wear always rapid and severe, which may further induce unexpected quality issues. However, cutting spindle power vibration signals change are complex, influenced by many factors due to different materials properties. The TCF stacks remains challenging, sensitive features difficult extract...

Transcriptional activation of the immediate early genes c-fos and egr-1 by extracellular signals appears to be mediated by ternary complex factors (TCFs). In BAC-1 macrophages, growth factor stimulation leads to the retardation of protein-DNA complexes containing distinct TCFs. One TCF is recognized by Elk-1 antisera, whereas the other is immunologically related to SAP-1. The appearance and dec...

PURPOSE The role of the Wnt[b]/beta-catenin-dependent pathway (canonical Wnt pathway) in the context of retinal development and homeostasis is largely unknown. This study was undertaken to characterize activation of the Wnt canonical pathway and its relevance to cell type populations in the developing and adult retina. METHODS Tissue from TCF/Lef-LacZ (T-cell-specific transcription factor/lym...

Aberrant activation of β-catenin/Tcf-4 signaling has been implicated in human carcinogenesis, including colorectal cancer. In this study, we compared the effects of Tcf-4 knockdown with β-catenin knockdown on cell proliferation, apoptosis, and chemosensitivity in SW480 and HCT116 colon cancer cells using adenoviral vector-mediated short hairpin RNA (shRNA). Our results show that, compared to β-...

A major outcome of the canonical Wnt/beta-catenin-signalling pathway is the transcriptional activation of a specific set of target genes. A typical feature of the transcriptional response induced by Wnt signalling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signalling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: in a...

T-cell factor (TCF)-1 protein forms the transcriptional complex with beta-catenin and regulates the expression of diverse target genes during early development and carcinogenesis. We have selected previously an RNA aptamer that binds to the DNA-binding domain of TCF-1 and have shown that it interfered with binding of TCF-1 to its specific DNA recognition sequences in vitro. As an approach to mo...

The ester functionality is commonly seen in the areas of chemical biology and medicinal chemistry for design cell-permeable active molecules. Ester-based pro-drug/pro-sensor strategies are employed to mask polar functional groups (i. e. carboxylic acids) improve overall cell permeability these However, their use as reactive units sensing applications, including bacterial detection, has not been...