An M13 phage deletion mutant, M13 delta E101, developed as a vector for selecting DNA sequences that direct DNA strand initiation on a single-stranded template, has been used for cloning restriction enzyme digests of phi X174 replicative-form DNA. Initiation determinants, detected on the basis of clear-plaque formation by the chimeric phage, were found only in restriction fragments containing t...

The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating...

A theoretical framework for predicting low frequency Raman vibrational spectra of viral capsids is presented and applied to the M13 bacteriophage. The method uses a continuum elastic theory for the vibrational modes and a bond-charge polarizability model of an amorphous material to roughly predict the Raman intensities. Comparison is made to experimental results for the M13 bacteriophage virus.

Although globular cluster horizontal-branch (HB) morphology is determined primarily by the cluster metallicity, observations have shown that clusters with similar metallicities sometimes have very different HB's, indicating the existence of a second parameter. Sweigart (1997, 1999) suggested that deep mixing on the red giant branch (RGB) may be a second parameter if He is mixed into the stellar...

The Multiple Meanings of Open Government Data: Understanding Different Stakeholders and Their Perspectives Felipe Gonzalez-Zapata & Richard Heeks Centre for Development Informatics, University of Manchester, Manchester, M13 9PL, UK Corresponding author: Prof. Richard Heeks, Centre for Development Informatics, IDPM, SEED, University of Manchester, Manchester, M13 9PL, UK, +44-161-275-2870 richar...

Hybrids composed of phage M13 and plasmid pHV33 were used to study the formation of deletions in Escherichia coli. Eighty to ninety percent of the deletion endpoints were at the position of the nick introduced into the M13 replication origin by the phage gene II protein. This suggests the existence of a novel mechanism of illegitimate recombination.

We found that parents of college students report greater goal-autonomy than their children, consistent with organismic, humanistic, and trait perspectives upon positive aging. Parents also had higher levels of positive aVect and lower levels of negative aVect than their children. In a second, retrospective test of the age-to-autonomy relationship, parents reported more autonomy now compared to ...

Building upon earlier observations which demonstrate substantial star-to-star differences in the carbon abundances of M13 subgiants, we present new Keck LRIS spectra reaching more that 1.5 mag below the M13 main-sequence turn-off (to V ≈ 20). Our analysis reveals a distribution of C abundances similar to that found among the subgiants, implying little change in the compositions of the M13 stars...