Ligand affinity chromatography was used to purify a cell surface alpha 2-macroglobulin (alpha 2M) receptor. Detergent extracts of human placenta were applied to an affinity matrix consisting of alpha 2M, previously reacted with methylamine, coupled to Sepharose. Elution with EDTA specifically released polypeptides with apparent molecular masses of 420 and 39 kD. In some preparations, small amou...

Human class I transplantation antigens are cell-surface-expressed molecules composed of one glycosylated, membrane-integrated heavy chain and one nonglycosylated, water-soluble subunit, beta 2-microglobulin (beta 2m). We have examined the intracellular transport of the two subunits by microinjecting mRNA into Xenopus laevis oocytes. Beta 2m, translated in oocytes, was transported and secreted i...

Cytotoxic T lymphocytes (CTL) can recognize antigenic peptides bound in a groove formed by the alpha 1 and alpha 2 domains of the heterodimeric major histocompatibility complex class I molecule. Proper assembly, transport, and stability of functional class I molecules is thought to require beta 2 microglobulin (beta 2m), the light chain of the class I heterodimer. We show here that the requirem...

A signed graph is a graph where the edges are assigned either positive ornegative signs. Net degree of a signed graph is the dierence between the number ofpositive and negative edges incident with a vertex. It is said to be net-regular if all itsvertices have the same net-degree. Laplacian energy of a signed graph is defined asε(L(Σ)) =|γ_1-(2m)/n|+...+|γ_n-(2m)/n| where γ_1,...,γ_n are the ei...

It is shown that the conclusions reached by Tereno are completely faulty We have recently shown that the Kruskal derivative assumes a form [1] du dv → f(r, t, dr/dt) ±f(r, t, dr/dt) (1) because u → ±v as r → 2M . Although this limit attains a value of ±1 irrespective of f → 0,∞, or anything, Tereno [2] refuses to accept this. Although, we have already pointed out that one should work out the li...

Beta 2-Microglobulin-deficient (beta 2m -/-) mice are reported to lack cell surface expression of major histocompatibility complex (MHC) class I molecules, CD8+ T cells, and the ability to mount MHC class I-specific T cell responses. We have observed that beta 2m -/- mice possess CD8+ T cells that can be induced to perform strong allospecific cytotoxic responses against nonself-MHC class I by i...

We investigate the relation between subleading soft graviton theorem and asymptotic symmetries in gravity even dimensions $d=2+2m$ higher than four. After rewriting as a Ward identity, we argue that charges of such identity generate Diff$(S^{2m})$. In order to show that, propose suitable commutation among certain components metric fields. As result, all Diff$(S^{2m})$ transformations are gravit...