Menurut para petani hal yang paling merugikan dalam produksi tanaman padi ini adalah hama dan penyakit, sering dialami oleh disebabkan karena adanya penyakit terdapat pada padi. Kurang nya pengetahuan tentang serta terbatas jumlah ahli menangani masalah tersebut sangat menghambat mencegah ada, maka dari itu perlu diabngun sebuah sistem pakar untuk mengidentifikasikan di Desa Purworejo. Penerapa...

To examine the effects of water stress and Cenangium ferruginosum (CF) on the fungal endophytic community of needles of Pinus koraiensis (PK), fungal endophytes isolated from the needles of 5-year-old PK seedlings were compared before and after exposure to water stress conditions and artificial inoculation with CF ascospores. Artificial CF inoculation was successfully confirmed using PCR with C...

Seven previously undescribed diaporthalean species were found in eastern Asia and eastern North America. Three of these new species and one previously known species are assigned to a new genus Leucodiaporthe. Descriptions and illustrations are provided for the new taxa including Allantoporthe leucoth6es sp. nov., Diaporthella corylina sp. nov., Leucodiaporthe acerina sp. nov., L. juglandis sp. ...

We extend detection of arthropod predator gut contents by polymerase chain reaction (PCR), heretofore restricted to insect predators, to spiders. Single individuals of the corn lead aphid, Rhopalosiphum maidis, were detected in the guts of spiderlings of Oxyopes salticus up to 12 h after feeding; individuals of the congeneric bird cherry oat aphid, R. padi, were not detected. Unfed O. salticus ...

Penelitian ini bertujuan untuk mengetahui interaksi antara varietas dan konsentrasi larutan ekstrak tauge terhadap vigor viabilitas benih padi, serta terbaik padi. Menggunakan Rancangan Acak Lengkap (RAL) dengan faktorial. Faktor pertama padi yaitu V1=Inpari 30, V2=Balimau, V3=Siam Epang faktor kedua K1=10%, K2=20%, K3=30% yang diulang sebanyak 3 (tiga) kali, sehingga diperoleh 27 satuan percob...

Saat ini perkembangan teknologi yang semakin meningkat memberikan pengaruh positif bagi kehidupan manusia, termasuk di bidang pertanian. Salah satunya adalah Sistem Pakar dapat mentransfer pengetahuan pakar ke dalam aplikasi komputer untuk mempermudah dan mempercepat mendiagnosis suatu kelainan atau penyakit pada tanaman padi. Minimnya tentang hama oleh petani terbatasnya jumlah tenaga ahli men...

Seiring dengan pertambahan jumlah penduduk tentu akan membutuhkan ketersediaan bahan pangan pokok masyarakat yang semakin meningkat pula. Padi sebagai salah satu komoditas utama Indonesia menjadi perhatian penting bagi pemerintah Indonesia. Menurut penilaian dari The Global Food Safety Initiative (GFSI) terjadi penurunan nilai indek ketahanan di sebesar 2.2% tahun 2021. Hal ini diikuti banyak d...

Detailed investigation of virus replication is facilitated by the construction of a full-length infectious clone of the viral genome. To date, this has not been achieved for members of the family Dicistroviridae. Here we demonstrate the construction of a baculovirus that expresses a dicistrovirus that is infectious in its natural host. We inserted a full-length cDNA clone of the genomic RNA of ...

Rhopalosiphum padi virus (RhPV) (family Dicistroviridae; genus Cripavirus) is an icosahedral aphid virus with a 10kb positive-sense RNA genome. To study the molecular biology of RhPV, identification of a cell line that supports replication of the virus is essential. We screened nine cell lines derived from species within the Lepidoptera, Diptera and Hemiptera for susceptibility to RhPV followin...

Peptidylarginine deiminase (PADI) enzymes are increasingly being associated with the regulation of chromatin structure and gene activity via histone citrullination. As one of the PADI family members, PADI1 has been mainly reported to be expressed in the epidermis and uterus, where the protein in keratinocytes is thought to promote differentiation by citrullinating filament proteins. However, th...