Sequential Candida glabrata isolates were obtained from the mouth of a patient infected with human immunodeficiency virus type 1 who was receiving high doses of fluconazole for oropharyngeal thrush. Fluconazole-susceptible colonies were replaced by resistant colonies that exhibited both increased fluconazole efflux and increased transcripts of a gene which codes for a protein with 72.5% identit...

Strain Genotype Source BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Open Biosystems W303 MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 doa4 (BY4741) MATa doa4::KanMX4 Open Biosystems MHY753 MATa his3-Δ200 leu2D1 ura3-52 lys2-801 trp1-63 ade2-101 [19] MHY754 MATa his3-Δ200 leu2D1 ura3-52 lys2-801 trp1-63 ade2-101 cim3-1 [19] YKA247 (W303) bar1::URA3 acm1::KanMX4 [11] YKA404 MATα acm1::Na...

We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3-O-acetyltransferase that is the F. sporotrichioides homolog of the Fusarium grami...

وراثت مقاومت سفیدبالک گلخانه، Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae)، به دو ترکیب ایمیداکلوپرید و کلرپایریفوس مورد مطالعه قرار گرفت. در این پژوهش، از جمعیت فیلستان ورامین (FL) عنوان والد مقاوم فردیس کرج (FR) حساس استفاده شد. مقدار ترتیب حدودا 62/13 91/14 برابر بود. عدم وجود اختلاف معنی‌دار LC50 روی تلاقی‌های F1 (R♂×S♀) Fʹ1 (R♀×S♂) نشان داد که T. نوع اتوزومی (غیرجنسی) است. ه...

Nagilactone E, an antifungal agent derived from the root bark of Podocarpus nagi, inhibits 1,3-β glucan synthesis; however, its inhibitory activity is weak. Anethole, principal component anise oil, enhances nagilactone E. We aimed to determine combinatorial effect and underlying mechanisms action E anethole against budding yeast Saccharomyces cerevisiae. Analyses using gene-deficient strains sh...

Proper execution of transcriptional programs is a key requirement of gene expression regulation, demanding accurate control of timing and amplitude. How precisely the transcription machinery fulfills this task is not known. Using an in situ hybridization approach that detects single mRNA molecules, we measured mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae ce...