The antibiotic kirromycin (Kr) inhibits bacterial protein synthesis by binding to elongation factor Tu (EF-Tu). Streptomyces cinnamoneus and Nocardia lactamdurans, producers of antibiotics of the Kr class, are known to possess an EF-Tu resistant to Kr. Both micro-organisms appear to possess a single tuf gene and we have characterized the one from S. cinnamoneus, which belongs to the tuf1 family...

I. PRELIMINARIES Time utility function (TUF) scheduling goes beyond the starttime-deadline notion to express tasks’ temporal constraints. The goal of the scheduler is to maximize system utility under the assumption that tasks aggregate a given amount of utility to the system as a function of when they execute. Work available in the literature include the characterization of time utility functio...

We consider the problem of scheduling exception handlers in real-time systems that operate under runtime uncertainties including those on execution times, activity arrivals, and failure occurrences. The application/scheduling model includes activities and their exception handlers that are subject to time/utility function (TUF) time constraints and an utility accrual (UA) optimality criterion. A...

The VMA4 gene encodes a 26.6-kDa hydrophilic polypeptide which exhibits 34% sequence identity with the E subunit of the vacuolar ATPase from bovine kidney microsomes. The chromosomal VMA4 gene was inactivated by a 171-base pair deletion followed by insertion of the URA3 gene within the coding sequence. Null vma4 haploid mutants are viable. However, their growth is considerably slowed down speci...

BACKGROUND Identification of Staphylococci to species level in veterinary microbiology is important to inform therapeutic intervention and management. We report on the efficacy of three routinely used commercial phenotypic methods for staphylococcal species identification, namely API Staph 32 (bioMérieux), RapID (Remel) and Staph-Zym (Rosco Diagnostica) compared to genotyping as a reference met...

OBJECTIVE We recently isolated and characterized endothelial-like CD31(+) cells derived from mouse embryonic stem (mES) cells and identified their transcriptome. The main objective of this study was to determine the functional relevance of the transcripts of unknown function (TUF) for vasculature development. METHODS AND RESULTS We selected 2 TUFs of more than 27 to study their role for blood...

In a survey in 2020, Salix babylonica trees displaying symptoms of phyllody, little leaves, and dieback were observed Xinjiang province China. Nested PCRs carried out using universal primer pairs for 16 S rRNA tuf genes, detected the presence phytoplasmas symptomatic trees, while no amplification was found symptomless trees. Blastn phylogenetic analyses showed that belong to species ‘Candidatus...

AIMS The goal of this study was to construct a single-tube multiplex molecular diagnostic assay using linear-after-the-exponential (LATE)-PCR for the detection of 17 microbial pathogens commonly associated with septicaemia. METHODS AND RESULTS The assay described here detects 17 pathogens associated with sepsis via amplification and analysis of gene-specific sequences. The pathogens and their...