Naturally occurring isothiocyanates, such as benzyl isothiocyanate (BITC), are potent and selective inhibitors of carcinogenesis induced by a variety of chemical carcinogens. These effects appear to be mediated through favorable modification of both phase I and II enzymes involved in carcinogen metabolism. The inactivation of rat and human cytochromes P450 (P450s) in microsomes and the reconsti...

Polycyclic aromatic hydrocarbons (PAHs) and heavy metals are often environmental cocontaminants that could interact to alter PAH carcinogenicity. The heavy metal, arsenite, and the PAH, benzo[k]fluoranthene, were used as prototypes to investigate, in human HepG2 cells, mechanisms whereby the bioactivation of benzo[k]fluoranthene by human CYP1A1 could be diminished by arsenite-mediated decreases...

Constitutive swine enzymes analogous to human/rat cytochrome P450 (CYP) isoforms 1A2, 2A6, 2B1/2/6, 2D6, 2E1, 3A1, and 4A1/3 were detected by Western blot analysis. Swine 2E1 has a molecular weight greater than rat 2E1; swine 2B2 has a molecular weight similar to human 2B6. An induction cocktail containing beta-naphthoflavone, phenobarbital, and dexamethasone induced immunoreactive homologs of ...

Alkyl-substituted polycyclic aromatic hydrocarbons may be metabolized to highly reactive benzylic sulfuric acid esters via benzylic hydroxylation and subsequent sulfonation. We have studied the benzylic hydroxylation of 1-methylpyrene (MP), a hepatocarcinogen in rodents, and 1-ethylpyrene (EP), whose benzylic hydroxylation would produce a secondary alcohol (alpha-HEP), in contrast to the primar...

Cytosolic sulfotransferases (SULTs) are responsible for the metabolism of a variety of drugs, xenobiotics, and endogenous compounds. Most cytosolic SULTs are found to be homodimers. However, transformation between monomeric and dimeric SULTs can be achieved by a single amino acid mutation. The importance of quaternary structure for cytosolic sulfotransferase was investigated using recombinant h...

HOLGER EBEL, JÖRN DAVIDSEN, AND STEFAN BORNHOLDT Institute of Theoretical Physics, Christian-Albrechts-University of Kiel, 24098 Kiel, Germany; e-mail: [email protected] Chemical Physics Theory Group, Department of Chemistry, University of Toronto, Toronto, Canada M5S 1A1; e-mail: [email protected] Interdisciplinary Center for Bioinformatics, University of Leipzig, Kreu...

Consider the random hyperbolic polynomial, f(x) = 1a1 coshx+···+np × an coshnx, in which n and p are integers such that n ≥ 2, p ≥ 0, and the coefficients ak(k = 1,2, . . . ,n) are independent, standard normally distributed random variables. If νnp is the mean number of real zeros of f(x), then we prove that νnp = π−1 logn+ O{(logn)1/2}.

©2015 Pulsus Group Inc. All rights reserved Respiratory Division, Department of Medicine, McGill University, Montreal, Quebec Correspondence: Dr James G Martin, Department of Medicine, A. 3.09, 687 Pine Avenue West,Montreal, Quebec H3A 1A1. E-mail [email protected] JG Martin, K Schwartzman. Respiratory medicine and research at McGill University: A historical perspective. Can Respir J 2015;...

We report here the sequences of two reference strains of parvovirus B19 (B19V) used for quantitation of B19V DNA. One reference strain has been established by the World Health Organization (WHO) and the other by the European Pharmacopeia (Ph. Eur.) and belong to B19V genotype 1a1 and 1a2, respectively.

Cytochrome P450 (P450)-dependent metabolism of all-trans-retinoic acid (atRA) is important for the expression of its biological activity. Because the human P450s involved in the formation of the principal atRA metabolites have been only partially identified, the purpose of this study was to identify the human P450s involved in atRA metabolism. The use of phenotyped human liver microsomes (n = 1...