Expression of the linear DNA plasmid-encoded protein (RS224) from the plant-pathogenic fungus Rhizoctonia solani isolate H-16, anastomosis group 2-2, and its localization were studied. Extracts from Escherichia coli cells expressing the open reading frame (ORF) of RS224 (RS224ORF in pRS224) contain a 92-kDa T7.Tag-RS224orf fusion protein. Antisera raised against the fusion protein obtained from...

A study was undertaken to explore the effect of volatile oils from Eucalyptus citriodora and its major constituent citronellal against two well-known rice pathogens, Rhizoctonia solani and Helminthosporium oryzae. The radial growth and dry weight of both the test fungi were drastically reduced in response to the volatile oils. A complete inhibition of R. solani and H. oryzae was observed at 10 ...

Bioassay-guided separation with an eye toward antifungal activity led to the isolation of the new alkaloid 5-(1̀,1̀-dimethylallyl)-8-hydroxyfuro[2-3-b] quinoline (1) and the known biscoumarin daphnoretin (2) as the active constituents of the chloroform extract obtained from the leaves of Ruta chalepensis. The structures of the metabolites were elucidated on the basis of their spectral characteris...

Rhizoctonia solani is a soil-borne fungus causing sheath blight. In consistent with its necrotrophic life style, no rice cultivars fully resistant to R. solani are known, and agrochemical plant defense activators used for rice blast, which upregulate a phytohormonal salicylic acid (SA)-dependent pathway, are ineffective towards this pathogen. As a result of the unavailability of genetics, the i...

From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC₅₀ of 30±4 µM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and w...

rhizoctonia solani is the most serious problem on sugar beet beta vulgaris l. grown in north dakota and minnesota. picoxystrobin, a quinone outside inhibitor, and penthiopyrad, a succinate dehydrogenase inhibitor, were used alone and in combinations for controlling r. solani ag 2-2 iiib on sugar beet under greenhouse conditions of 22 ± 2 °c and a 12-h photoperiod. fungicides were applied in-fur...

A series of pyrazole carboxamide and isoxazolol pyrazole carboxylate derivatives were designed and synthesized in this study. The structures of the compounds were elucidated based on spectral data (infrared, proton nuclear magnetic resonance and mass spectroscopy). Then, all of the compounds were bioassayed in vitro against four types of phytopathogenic fungi (Alternaria porri, Marssonina coron...

Seed health testing by studying the seed-borne fungi of soybean seeds is an important step in the management of the seed-borne diseases. In the present study seed-borne fungi was studied by using agar plate method. A number of seed-borne fungal species was isolated from the agar plate. These seed-borne fungal sps. were Aspergillus niger, A. flavus, A. fumigatus, Chaetomium globosum, Curvularia ...

Biocontrol of Rhizoctonia solani in tomatoes cultivated under greenhouse and field conditions was analyzed using the Trichoderma harzianum mutants Th650-NG7, Th11A80.1, Th12A40.1, Th12C40.1 and Th12A10.1 and ThF2-1, respectively. Their innocuousness on tomato cultivars 92.95 and Gondola (greenhouse assays), and on cultivar Fortaleza (field assays) was established. Alginate pellets (1.7 g pellet...