RATIONALE During exercise, heart failure patients (HF) show an out-of-proportion ventilation increase, which in patients with COPD is blunted. When HF and COPD coexist, the ventilatory response to exercise is unpredictable. OBJECTIVES We evaluated a human model of respiratory impairment in 10 COPD-free HF patients and in 10 healthy subjects, tested with a progressive workload exercise with di...

BACKGROUND Ulinastatin (UTI) is a urinary trypsin inhibitor extracted and purified from urine of males. This study aimed to explore the effects of UTI on paraquat-induced-oxidative stress in human type II alveolar epithelial cells. METHODS The human type II alveolar epithelial cells, A549 cells, were cultured in vitro. The A549 cells were treated with different concentrations of paraquat (200...

IJABELEI) URIC ACTI) (as a 0.50 c./ml. solution in dilute fornmalin) and glucose (as a 5.0 .tc./ml. solution in saturated henzoic acid) were used. Tlmese were diluted with distilled water, and 0.20-mi. aliquots were placed in the bottom of 25-mm. test tui)es om25-nil. Erlenmeyer flasks and evaporated to dryimess. Dilutions were calculated to keep counting rates between 4000 and 2 ,000 cprn. As ...

Glatiramer acetate (GA; Copaxone, Τeva Pharmaceuticals) is an injectable peptide mixture with immunomodulatory properties. It is approved by the FDA as a first-line treatment for reducing the frequency of relapse and slowing the progression of disability in the relapsing-remitting form of multiple sclerosis (MS) [1]. A considerable amount of research conducted over the last decade has demonstra...

The relationship between the release of platelet activating factor (PAF), leukotriene C(4)/D(4)/E(E) (LTC(4)/D(4)/E(4)) and prostaglandin D(2) (PGD(2)) from nasal mucosa in vivo was examined in 24 rhinitis patients allergic to the house dust mite (HDM). During a double blind placebo controlled cross-over study 200 mug fluticasone propionate aqueous nasal spray (FPANS) was administered twice dai...

Objective: The primary purpose of this research was to develop a simple, precise, fast, and accurate method for measuring cefoperazone sulbactam simultaneously in dried blood spots (DBS) using HPLC PDA. Methods: A simplified analytical quantifying DBS samples High-Performance Liquid Chromatography photodiode array detector with isocratic elution developed validated. best chromatographic conditi...

Heroin is an illegal drug and monitoring heroin use is important in rehabilitation, pain management services, and workplace employment testing. In humans, heroin is rapidly converted to 6-acetylmorphine (6AM), which is quickly hydrolyzed to morphine. Morphine glucuronide is the major metabolite of heroin in urine (1 ). However, 6AM is a unique metabolite of heroin and is considered the definiti...

Adverse reactions to iron dextran are usually delayed and consist of joint pain, vomiting, nausea, headache, and fever. Lifethreatening reactions to iron dextran have been reported [1,2]. We report the case of a 48-year-old woman who experienced a severe reaction to iron dextran and who was able to receive the drug after undergoing a series of tolerance induction protocols. The patient had celi...

Methods for extracting extrachromosomal DNA from eukaryotic cells are important for many studies such as DNA replication and identification of certain viruses (2). The procedure described by Hirt (4), which has been widely used over the last thirty years, is laborious and time-consuming and requires phenol extraction. More rapid methods using anion-exchange chromatography (5) or silicon-based r...