A glutathione-S-transferase was isolated and purified to homogeneity from human platelets. With a combination of ammonium sulfate fractionation and chromatographic methods, 0.2 mg of pure enzyme was obtained from 9 X 10(11) platelets with a 12% recovery. The purified enzyme had a specific activity of 7.5 U per milligram, representing an approximately 1,100-fold purification. The enzyme was foun...

The title compound, C(12)H(9)N(2) (+)·C(7)H(4)ClO(3) (-)·C(12)H(8)N(2)·C(7)H(5)ClO(3), contains one phenanthrolinium (Hphen) cation, one phenanthroline (phen) mol-ecule, one 4-chloro-2-hydroxy-benzoate anion (hcba) and one 4-chloro-2-hydroxy-benzoic acid (Hhcba) mol-ecule in the asymmetric unit. The phen mol-ecule is approximately parallel to Hphen, making a dihedral angle of 1.98 (6)°. The cen...