Embryonic stem cells can differentiate in vitro into hematopoietic cells through two intermediate stages; the first being FLK11 E-cadherin2 proximal lateral mesoderm and the second being CD452 VE-cadherin1 endothelial cells. To further dissect the CD452 VE-cadherin1 cells, we have examined distribution of a4-integrin on this cell population, because a4-integrin is the molecule expressed on hema...

28-hydroxymilbemycins, 1 3/?,30-dihydroxymilbemycins, and others.4~8) On the other hand, Ramos Tomboet al. have reported 13/Miydroxylation and 14, 1 5-epoxidation of milbemycins by Streptomyces violascens ATCC31560.9) During our screening of microbial conversion of milbemycin A4 (la), we observed that an actinomycete, strain SANK65087, newly isolated from soil, converted milbemycin A4 (la) to a...

A sequence encoding a novel glutathione transferase, GST A4-4, has been identified in a human fetal brain cDNA library. The protein has been produced in Escherichia coli after optimization of the codon usage for high-level heterologous expression. The dimeric protein has a subunit molecular mass of 25704 Da based on the deduced amino acid composition. Human GST A4-4 is a member of the Alpha cla...

argumentation frameworks [Dun95] take a very simple view on argumentation as they do not presuppose any internal structure of an argument. Abstract argumentation frameworks only consider the interactions of arguments by means of an attack relation between arguments. Definition 1 (Abstract Argumentation Framework). An abstract argumentation framework AF is a tuple AF = (Arg,→) where Arg is a set...

Comparative analysis of the inducible elements of the mouse interferon A4 and A11 gene promoters (IE-A4 and IE-A11) by transient transfection experiments, DNase 1 footprinting and electrophoretic mobility shift assays resulted in identification of a virus-induced binding activity suggested to be involved in NDV-induced activation of transcription of these genes. The virus-induced factor, termed...

Microbial oxidation of milbemycin A4 at the C-25 ethyl group was performed. Milbemycin A4 was converted to 31- and 32-hydroxy derivatives by Circinella umbellata SANK 44272 along with 24- and 30-hydroxy derivatives. Related compounds, 5-ketomilbemycin A4 5-oxime and 13 beta-fluoromilbemycin A4 were similarly converted to the hydroxylated compounds by this microorganism. Absidia cylindrospora SA...

and Applied Analysis 3 Thus, the origin of system (13) is an element critical point. It could be investigated using the classical integral factor method. Now, we consider the following system: dx dt = y + A30x3n + A21x2ny + A12xny2 + A03y3, dy dt = −x2n−1 + xn−1 (B30x3n + B21x2ny + B12xny2 + B03y3) . (14) When n = 2k + 1, by those transformations, system (14) is changed into dx dt = −y − √2k + ...

Alfalfa mosaic virus (AlMV) RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs) have been previously shown to be encapsidated into virions by coat protein (CP) expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 are not required for virion assembly. Here, we constructed various mutants by deleting sequences within the 3' NTR of AlMV subgenomic (sg) RNA4 (same as o...