Real-Time PCR (qPCR) testing is recommended as both a diagnostic and outcome measurement of etiological treatment in clinical practice and clinical trials of Chagas disease (CD), but no external quality assurance (EQA) program provides performance assessment of the assays in use. We implemented an EQA system to evaluate the performance of molecular biology laboratories involved in qPCR based fo...

BACKGROUND Previous studies of intracellular expression of phospho-epitopes in human leukocytes using flow cytometry have used erythrocyte removal or lysis before fixation. Because many of the phospho-epitopes of interest are part of signaling networks that respond to the environment and turn over rapidly, the interval and manipulations used to eliminate erythrocytes from samples have the poten...

The present study was aimed to assess the incidence of T. evansi infections in camel Saurashtra region Gujarat, India, through microscopy and polymerase chain reaction (PCR) methods. Blood samples were collected from 192 camels, randomly. diagnostic sensitivity specificity Field’s stain, Giemsa stain RoTat1.2 assays for detection blood evaluated. infection recorded camels based on PCR 3.23%, 8....

Transmissible spongiform encephalopathies (TSEs) are zoonotic fatal neurodegenerative diseases in animals and humans. TSEs are commonly known as bovine spongiform encephalopathy in cattle, scrapie in sheep and goats, chronic wasting disease in cervids, and Creutzfeldt-Jakob disease in humans. The putative transmissible agents are infectious prion proteins (PrP(Sc)), which are formed by the conv...

background: q fever is an important widespread reemerging zoonosis. the presence of coxiella burnetii in 100 tick-infested dogs was assessed in this study. methods: the blood samples from 100 referred dogs were acquired and evaluated by nested-pcr. results: c. burnetii was detected in 11 out of 100 (11%) blood samples. most of the positive dogs were kept outdoor and fed on raw diet. based on ou...

background :  q fever is an important widespread reemerging zoonosis. the presence of coxiella burnetii in 100 tick-infested dogs was assessed in this study. methods :  the blood samples from 100 referred dogs were acquired and evaluated by nested-pcr. results :   c. burnetii was detected in 11 out of 100 (11%) blood samples. most of the positive dogs were kept outdoor and fed on raw diet. base...

a molecular survey was performed for identification theileria spp in sheep and ticks during from 2010-2011 in fasa and kazeroun areas, fars province, iran. a total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. the prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...

Sucheta Bansal, S. K. Joshi 1-DEPARTMENT OF ORAL AND MAXILLOFACIAL PATHOLOGY; 2DEPARTMENT OF PROSTHODONTICS INCLUDING CROWN AND BRIDGE & IMPLANTOLOGY, HIMACHAL INSTITUTE OF DENTAL SCIENCES PAONTA SAHIB, HIMACHAL PRADESH, INDIA Summary As a diagnostic fluid, saliva offers many advantages over serum especially in forensic science as it is good source for DNA retrieval which could be used for indi...

We measured urinary kallikrein by its esterolytic and kinin-forming activity in 5-minute urine samples obtained throughout continuous bleeding experiments in pigs to correlate possible changes in urinary kallikrein excretion during hemorrhagic hypotension with resin activity and renal cortical blood flow. Renin activity was determined in venous blood samples and renal cortical blood flow was es...

BACKGROUND Human immunodeficiency virus (HIV) Rapid diagnostic Test (RDT) kits are the preferred assays for HIV testing in many countries. Prevention of Mother-to-Child Transmission, Know Your Status Campaigns, Blood-Safety, Voluntary Counseling and Testing are major strategies adapted to control transmission of the virus and the pivot of these interventions is either screening or diagnosing in...