The objective of the present study was to isolate and identify Clostridium chauvoei from swab (n=750) and tissue (n=50) samples from cattle suspected for black quarter in six districts belonging to different climatic zones (Punjab, Pakistan). Conventional culture methods showed 192(25.6%) and 19(38%) positive from swab and tissues respectively for this bacterium. These bacterial isolates were f...

An obligate intracellular bacterium was isolated from urine samples from 7 (3.5%) of 202 fruit bats (Eonycteris spelaea) in peninsular Malaysia. The bacterium produced large membrane-bound inclusions in human, simian, and rodent cell lines, including epithelial, fibroblastlike, and lymphoid cells. Thin-section electron microscopy showed reticulate bodies dividing by binary fission and elementar...

Article Information Brinjal is an important vegetable crop grown worldwide. A field survey conducted during 2012 in Kerala state of South India, the little leaf disease affected brinjal plant was observed in one of the home gardens located at Nemmara (Palakkad district). The diseased and healthy samples were collected; genomic DNA was isolated and 16SrRNA gene amplified by performing nested PCR...

The damselfish genus Dascyllus comprises nine species of both largeand small-bodied fishes distributed over the entire Indo-West Pacific. Most members of the genus have polygynous mating systems with protogynous sex change, while others are promiscuous with no sex change. Hypotheses linking presumed phylogenetic relationships with body size, sex change and mating structure have been proposed pr...

OBJECTIVE Conventional diagnosis of bacterial vaginosis contains some controversial points. To understand accurately the relationship between clinical stages and the microbiotas, the intravaginal microbial flora was analyzed by the clone library method. STUDY DESIGN Vaginal fluid samples from 31 patients were examined. Lactobacillary grade, Nugent score, culture-based method, and clone librar...

Bioluminescence is a phenomena in an organism which produces luminescence on quorum sensing and this phenomenon has been explored in many fields like invivo diagnostics, marker technology, protein engineering etc. and has immense potential as future torchlight too with the aid of technologies. Present study aims at the screening and identification of bioluminescent bacteria from different marin...

To gain information about the relative proportion of introduced anguillid eel species inhabiting Japa nese natural waters, 98 eels were collected and genetically identified — 46 from the Uono River, Niigata, Japan, during their downstream spawning migration, and 52 silver eels from the East China Sea. Species identification using RFLP analysis of the mitochondrial 16SrRNA domain suggested that ...

Globally, hemotropic Mycoplasma spp. are emerging or re-emerging zoonotic pathogens that affect livestock, wildlife, companion animals, and humans, potentially causing serious and economically important disease problems. Little is known about hemotropic Mycoplasma spp. prevalence, host-specificity, or route of transmission in most species, including wildlife. DNA amplification by PCR targeting ...

Salmonella entericaserovar Typhi remains a major public health problem in many developing countries .The severity and pathogenesis of the disease depending on virulence factors. Previously, different virulence factors have been detected phenotypically and serologically for clinical and epidemiological investigation of salmonella. Beyond the phenotypic characterization, a reliable molecular meth...