Multiple signal transduction pathways mediating gonadotropin-induced testosterone and 17β-estradiol (E(2)) production were identified in carp ovarian theca and granulosa cells in short-term co-incubation. Inhibitors of voltage-sensitive calcium channels (VSCCs) and calmodulin attenuated human chorionic gonadotropin (HCG)-induced steroid production, whereas modulators of adenylate cyclase and pr...

Dehydroepiandrosterone (DHEA) has a fat-reducing effect, while little information is available on whether DHEA regulates glucose metabolism, which would in turn affect fat deposition. To investigate the effects of DHEA on glucose metabolism, rats were administered a high-fat diet containing either 0 (HCG), 25 (HLG), 50 (HMG), or 100 (HHG) mg·kg-1 DHEA per day via gavage for 8 weeks. Results sho...

Tumor hypoxia modifies the efficacy of conventional anticancer therapy and promotes malignant tumor progression. Human chorionic gonadotropin (hCG) is a glycoprotein secreted during pregnancy that has been used to monitor tumor burden in xenografts engineered to express this marker. We adapted this approach to use urinary beta-hCG as a secreted reporter protein for tumor hypoxia. We used a hypo...

The unsatisfactory standardization of many immunoassays is fairly well recognized, but the problem is hard to tackle (1 ). Recently, studies on the performance of immunoassays for testosterone in female serum (2 ) and for urinary cortisol (1 ) have revealed that the quality of some widely used assays is totally unsatisfactory. The quality of assays for human chorionic gonadotropin (hCG) has als...

High-frequency uterine contractions at the time of non-cavitating embryo transfer influence adversely IVF-embryo transfer outcome. This prompted us to quantify prospectively the possible decline in uterine contraction frequency occurring during later stages of the luteal phase of ovarian stimulation, up to the time of blastocyst transfers, in 43 IVF-embryo transfer candidates. Contractility was...

OBJECTIVES To evaluate the efficacy of hCG therapy on retractile testis in various testicular locations and age groups. SETTING AND DESIGN This study comprised 123 boys whose diagnosis were retractile testis with scrotal base, high scrotal and superficial inguinal localizations with an average age of 4,2 (1-8) years. METHODS 123 boys with retractile testes were given a total dose of 9000 hC...

The impact of human chorionic gonadotropin (hCG) on prostate carcinoma viability was investigated. Treatment of LNCaP and PC-3 cells with hCG modestly reduced cell viability within 96 h. Treatment of cells with hCG followed by exposure to ionizing radiation enhanced radiosensitivity. Exposure of LNCaP cells to hCG promoted activation of epidermal growth factor receptor (ERBB1) via a Galpha(i)-,...

Two experiments were conducted to determine farrowing rates in sows after estrus and ovulation were controlled by the use ofgonadotropins and prostaglandin F2a (PGF2a). Accessory corpora lutea (CL) were induced in 74 Yorkshire and Duroc sows by an injection of 1,500 IU of pregnant mare serum gonadotropin (PMSG) followed 72 hr later by 750 IU of human %horionic gonadotropin (HCG). Thirtyeight so...

nancy by the developing embryo after conception and later by the syncytiotrophoblast to prevent disintegration of the corpus luteum of the ovary. hCG also plays a role in cellular differentiation/proliferation and may activate apoptosis (1). hCG is extensively used as a parenteral fertility medication in lieu of luteinizing hormone. hCG is composed of 244 amino acids with a molecular mass of 36...

We have previously demonstrated that the release of arachidonic acid (AA) from human chorionic gonadotropin (hCG)-stimulated Leydig cells occurs in a dose- and time-dependent manner. In addition, the amount of AA released was dependent on the hormone-receptor interaction and the concentration of LH-hCG binding sites on the cell surface. The present study was conducted to evaluate the involvemen...