The conformationally restricted adenine nucleotide analogs 8,3'anhydro-8-oxy-9-(beta-D-xylofuranosyl)adenine-5'-O-tri(di)-phosphate (I), and 8,2'-anhydro-8-oxy-9-(beta-D-arabinofuranosyl) adenine-5'-O-tri(di)-phosphate (II), were prepared chemically as their alpha-32P-labelled compounds and compared with syn-structured 8-bromo AT(D)P in mitochondrial adenine nucleotide translocation. The experi...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Phosphorylation of the insulin-regulatable glucose transporter (IRGT) is increased by incubating rat adipocytes with isoproterenol or by incubating microsomal membranes with cAMP-dependent protein kinase. To attempt to locate the sites of phosphorylation, the IRGT (apparent Mr = 46,000) was immunoprecipitated from 32P-labeled adipocytes and cleaved with CNBr or trypsin. Essentially all of the 3...

Protein kinases (Rabinowitz, 1962) catalysing the transfer of the terminal phosphate of ATP to phosvitin, casein and possibly other phosphoproteins have been found in mammalian liver (Burnett & Kennedy, 1954), mammary gland (Sundararajan, Sampath-Kumar & Sarma, 1960), erythrocyte membranes (Judah, Ahmed & McLean, 1962) and brain (Rabinowitz & Lipmann, 1960). The following study of a protein kin...

Introduction Pterygium is a wing-shaped, vascular, fleshy growth that originates from the conjunctiva and can spread into the corneal limbus and beyond. Beta irradiation after bare sclera surgery of primary pterygium is a simple, effective, and safe treatment, which reduces the risk of local recurrence. Materials and Methods Dosimetric components of strontium-90 (90Sr), phosphorous-32 (32P), an...

The effects of protein phosphorylation and dephosphorylation on glucose transport activity reconstituted from adipocyte membrane fractions and its relationship to the phosphorylation state of the adipose/muscle-type glucose transporter (GLUT4) were studied. In vitro phosphorylation of membranes in the presence of ATP and protein kinase A produced a stimulation of the reconstituted glucose trans...

Incorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition of glucose to a cell suspension incubated with (32P)orthophosphoric acid. The 32P counts were coincident with the enzyme band when immunoprecipitates were subjected to sodium dodecyl sulfate disc gel electrophoresis. The incorporation of phosphate was associated with a decrease in enzyme activi...