The effect of norepinephrine on the Na+-K+ pump was investigated by simultaneously measuring intracellular sodium ion activity (aiNa) and contractile force of canine cardiac Purkinje fibers driven at 1.0 Hz in K+-free solution, high K+ solution, and in the presence of tetrodotoxin. In Tyrode solution containing 5.4mM [K+]o, 10(-6) M norepinephrine decreased aiNa, whereas in K+-free solution 10(...

0 2 4 6 8 Time of incubation at 30°C in shaking water bath (h) Fig. 1. Survival of cytochrome P-450 when shaken aerobically at 30°C in 0.1 M-potassium phosphate bufler: protection by chloramphenicol, cycloheximide, dinitrophenol and semianaerobic conditions Symbols: @ 0.1 M-phosphate buffer + 20% glucose + 1 mMchloramphenicol; A, 0.1 M-phosphate buffer + 20% glucose + 1 m~-2,4-dinitrophenol; 0,...

Introduction: It is intriguing that the majority of lunar meteorites (~30) have been discovered in the hot deserts of Middle-East and North Africa. In contrast, of 10s of thousands of meteorite finds from Antarctica over 30 years, only a handful (~5) are lunar. This bias towards the lunar meteorite recovery from the hot deserts may be due to the preferential fall of lunar meteorites in these re...

1. Intact WI-38 human diploid lung fibroblasts were shown to respond to epinephrine (3 X lop7 M) and prostaglandin El (PGE1) (0.57 X lop7 M) with increased cyclic adenosine 3’: 5’-monophosphate (cyclic AMP) levels. Corticotropin (1.25 units per ml), insulin (0.02 to 200 milliunits per ml), and glucagon (5 X lo-’ M) were ineffective. Although the response to PGE, was not clearly maximal at conce...

1. Intact WI-38 human diploid lung fibroblasts were shown to respond to epinephrine (3 X lop7 M) and prostaglandin El (PGE1) (0.57 X lop7 M) with increased cyclic adenosine 3’: 5’-monophosphate (cyclic AMP) levels. Corticotropin (1.25 units per ml), insulin (0.02 to 200 milliunits per ml), and glucagon (5 X lo-’ M) were ineffective. Although the response to PGE, was not clearly maximal at conce...

CDP-1,2-diacyl-sn-glycerol (CDP-diacylglycerol):myo-inositol phosphatidyltransferase (EC 2.7.8.11, phosphatidylinositol synthase) catalyzes the final step in the de novo synthesis of phosphatidylinositol in the endoplasmic reticulum fraction of germinating soybeans (Glycine max L. var Cutler 71). A variety of solubilization agents were examined for their ability to release phosphatidylinositol ...

1. Intact WI-38 human diploid lung fibroblasts were shown to respond to epinephrine (3 X lop7 M) and prostaglandin El (PGE1) (0.57 X lop7 M) with increased cyclic adenosine 3’: 5’-monophosphate (cyclic AMP) levels. Corticotropin (1.25 units per ml), insulin (0.02 to 200 milliunits per ml), and glucagon (5 X lo-’ M) were ineffective. Although the response to PGE, was not clearly maximal at conce...

The phase variation of the superconducting gap over the (normal) Fermi surface of the high-temperature superconductors remains a significant unresolved question. Is the phase of the gap constant, does it change sign, or is it perhaps complex? A detailed answer to this question would provide important constraints on various pairing mechanisms. Here we propose a new method for measuring the relat...

The toxin-binding protein of sugarcane susceptible to eyespot disease also possesses raffinose-binding activity. The K(m)'s for binding are: helminthosporoside (toxin) 6.8 x 10(-5) M, raffinose 2.5 x 10(-5) M, and melibiose 2.6 x 10(-5) M. Evidence obtained by administering [(14)C]raffinose to sugarcane protoplasts suggests that this protein participates in alpha-galactoside transport. Cells fr...