lean thinking requires certain techniques and tools to support lean processing and to penetrate an organization in which 5s system (sorting, set in order, shining, standardization, sustaining) is to develop value process workflow system. kanban, a card containing a set of manufacturing specifications and requirements used to regulate the supply of components, is one of the most famous and impor...

Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y ...

This pattern can be explained by assuming that the ancestral, eukaryotic 5S rRNA shared the first two features with the archaebacterlal and eubacterial 5S rRNAs, and that these were altered in the eukaryotic branch of evolution only after the divergence of the red algae, the latter conserving these ancestral features until the present time. As such, the study of 5S rRNA secondary structure cons...

A model for protein synthesis is proposed in which the donor for the peptide elongation reaction is peptidyl-5S RNA. Space-filling models show that peptide bond formation between peptidyl-5S RNA and aminoacyl-tRNA is eminently feasible from a stereochemical point of view. The peptide is transferred to 5S RNA, while at the same time the deacylated tRNA is exchanged by a new aminoacyl-tRNA accept...

The nucleotide sequences of 5S rRNA from two red algae, Gracilaria compressa and Porphyra tenera have been determined. The two 5S rRNAs are fairly dissimilar to each other in their sequences (65% identity), although they are both composed of 121 nucleotides. Their secondary structures are generally of the eukaryotic with a prokaryotic characteristic. Judged from the 5S rRNA sequence data, the r...

Incorporation of somatic histone H1 into chromatin during embryogenesis of Xenopus laevis results in repression of transcription of the oocyte- but not the somatic-type 5S rRNA genes. We showed earlier that a similar effect of the H1 observed in chromatin reconstituted on circular plasmids in vitro depends on its binding to the AT-rich flanks of the oocyte-type 5S rRNA gene. H1 binding results ...

We calculate the decay rate ratios for OZI allowed decays of Υ(5S) to two B mesons by using the decay amplitudes which incorporate the wave function of the Υ(5S) state. We obtain the results that the branching ratio of the Υ(5S) decay to B∗ sB ∗ s is much larger than the branching ratio to BsB ∗ s or BsB ∗ s , in good agreement with recent experimental results of CLEO and BELLE. This agreement ...

The 5S ribosomal RNA (rRNA) genes in eukaryotes may occur either interspersed with the other rRNA genes in the ribosomal DNA (rDNA) repeat, or in separate tandem arrays, or dispersed throughout the genome. In Pythium species and in several related Oomycetes, polymerase chain reaction (PCR) amplification of the nontranscribed spacer (NTS) region with one primer specific for the 5S gene revealed,...

Ribosomes containing 5S RNA in which the 3'-terminus has been modified chemically, either by periodate oxidation or by conversion of the terminal ribose to a morpholine derivative, are fully as active in peptidebond formation and polypeptide synthesis as those ribosomes with unmodified 5S RNA. These results exclude models for protein synthesis in which peptidyl-5S RNA is formed as an intermediate.