Compound C2 is a product of the reaction of O2 and the mixed-valence state of cytochrome oxidase. The mixed-valence state of membrane-bound cytochrome oxidase is obtained at -24 degrees C, by using either ferricyanide or yeast peroxidase complex ES as oxidants, and the configurations of oxidized haem a and its associated copper (a3+Cua2+) and of reduced haem a3 and its associated copper (ac3+.C...

Cutaneous fibrosis after wound is evoked by myofibroblasts capable of producing collagen; the derivation and features remain to be investigated. Immunophenotypical characteristics of myofibroblasts were analysed in excisional rat wound healing, of which samples were obtained on post-wounding (PW) days 1 to 26. Myofibroblasts were characterized for expressions of intermediate cytoskeletons such ...

The present study investigates mRNA and protein levels of A3 adenosine receptors in resting (R) and activated (A) human lymphocytes. The receptors were evaluated by the antagonist radioligand [3H]5-N-(4-methoxyphenyl-carbamoyl)amino-8-propyl-2(2furyl)-pyrazolo-[4,3e]-1,2,4-triazolo-[1,5-c]-pyrimidine ([3H]MRE 3008F20), which yielded Bmax values of 125 +/- 15 and 225 +/- 23 fmol/mg of protein an...

In recent work on the reaction between oxygen and reduced cytochrome oxidase made by the method of Yonetani (1)) Gibson and Greenwood (2) found that the reaction is complex and that at least two spectroscopically distinguishable reactions must take place. The kinetic changes were interpreted in terms of a scheme proposed by Keilin and Hartree (3), according to which cytochrome oxidase preparati...

In recent work on the reaction between oxygen and reduced cytochrome oxidase made by the method of Yonetani (1)) Gibson and Greenwood (2) found that the reaction is complex and that at least two spectroscopically distinguishable reactions must take place. The kinetic changes were interpreted in terms of a scheme proposed by Keilin and Hartree (3), according to which cytochrome oxidase preparati...

A primary role of GSTs (glutathione transferases) is detoxication of electrophilic compounds. In addition to this protective function, hGST (human GST) A3-3, a member of the Alpha class of soluble GSTs, has prominent steroid double-bond isomerase activity. The isomerase reaction is an obligatory step in the biosynthesis of steroid hormones, indicating a special role of hGST A3-3 in steroidogeni...

the application of the [pd{c6h3(ch2ch2nh2)-4-ome-5-κ2-c,n}(μ-br)]2 complex of 2-methoxyphenethylamine in the heck coupling reaction was considered under both conventional and microwave irradiation conditions, and their results were compared. this complex is an efficient, stable and non-sensitive to air and moisture catalyst for the vinylation of substituted aryl halides with different electroni...

We discuss an autocatalytic reaction system: the cyclic competition model A1 + A2 → 2A2, A2 + A3 → 2A3, A3 + A4 → 2A4, A4 + A1 → 2A1), as well as its neutral counterpart. Migrations are introduced into the model. When stochastic phenomena are taken into account, a phase transition between a “fixation” and a “neutral” regime is observed. In the “fixation” regime, species A1 and A3 form an allian...