Barrel-ageing of conventionally fermented beers is becoming increasingly popular in recent years, but only very little known about the underlying process. In this study, we show that wood species significantly affects bacterial community composition, beer chemistry and sensory characteristics throughout 38 weeks barrel-ageing. Whereas microbial communities oak- acacia-aged became dominated by P...

A novel species of acetic acid bacteria belonging to the genus of Acetobacter, to which a scientific name of Acetobacter altoacetigenes MH-24 (FERM BP-491) is given. The bacteria can grow in a culture medium con taining at least 4 w/v. 9% of acetic acid with a pH of 3.5 at the highest. Pure samples of species have been iso lated and used for the fermentation production of vine gar without under...

Microbial terminal oxidases are usually associated with particulate fractions in the cell. These fractions, however, are difficult to resolve into smaller active molecules that are suitable for enzymatic studies in vitro. This fact led Rittenberg (1) to propose that these particles are surrounded by a special membrane. The present paper will report a method of “solubilization”’ of particulate o...

Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. trop...

Here we report the 3.7-Mb draft genome sequence of Acetobacter tropicalis NBRC16470(T), which can produce optically pure d-glyceric acid (d-GA; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes.

A novel carbonyl reductase (AcCR) catalyzing the asymmetric reduction of ketones to enantiopure alcohols with anti-Prelog stereoselectivity was found in Acetobacter sp. CCTCC M209061 and enriched 27.5-fold with an overall yield of 0.4% by purification. The enzyme showed a homotetrameric structure with an apparent molecular mass of 104 kDa and each subunit of 27 kDa. The gene sequence of AcCR wa...