Androgens produced by stimulating mouse testis with gonadotropic hormones cause a rise in renal beta-glucuronidase but not an increase in acid or alkaline phosphatase. All subcellular components increase in beta-glucuronidase activity, with a relatively greater increment in particulate enzyme as compared with that free in the cytoplasm (non-sedimentable). A small percentage of recovered beta-gl...

Acid phosphatase activity is determined by splitting 1-naphthyl phosphate, concurrently diazotizing the released 1-naphthol with Fast Red TR, and measuring the resulting color. The test is performed in the presence and absence of tartrate. Reaction rates can be continuously monitored, and their difference is proportional to acid phosphatase activity that is inhibited by tartrate. Results for se...

This specific phosphatase is a 5-nucleotidase; it differs from the alkaline phosphatase in its range of action and in its optimal pH. Whereas alkaline phosphatase, which is non-specific, hydrolyses a great variety of phosphoric esters, the 5-nucleotidase hydrolyses only nucleotides with the phosphate group attached to the fifth carbon of the pentose, that is, muscle adenylic acid (adenosine-5-m...

Acid phosphatase activity increases above normal in the liver of whole body irradiated animals. It is considered to be one of the adverse radiation effects. Increase in the acid phosphatase activity was dose dependent. Centella asiatica extract, which increased the survival of gamma irradiated Swiss albino mouse when given prior to irradiation was tested to observe changes in the acid phosphata...

Increased acid phosphatase activity in the serum and tissues of patients with Gaucher's disease has now been recognized for two decades, but as yet no relation has been established between the enzyme and the etiology and progress of the disease. Here, we review results obtained by various investigators, ranging from a consideration of the methods used for the evaluation of serum acid phosphatas...

UNLABELLED The presence of semen in vaginal fluid, as identified by an acid phosphatase spot test, does not influence vaginal proinflammatory cytokine concentrations. OBJECTIVE Determine whether semen, as detected by acid phosphatase, influences vaginal cytokines or secretory leukocyte protease inhibitor concentrations. METHODS 138 pregnant women had vaginal fluid collected for Gram stain, ...

The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase...

Lysosomal enzymes acid phosphatase show increase in diabetes mellitus and this is closely related to diabetic metabolic alterations. This was a crosssectional study conducted on 50 diabetic subjects (25 patients with complications and 25 patients without complications) in RIMS hospital during the period from September 2010 to August 2012. Twenty five age and sex matched healthy individual were ...

An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange chromatography on DEAE cellulose. The acid phosphatase gave a single band on SDS-polyacrylamide slab gel electrophoresis. The molecular weight of the aci...