The hyperthermophilic archaeon Acidianus ambivalens expresses a membrane-bound aa(3)-type quinol oxidase, when grown aerobically, that we have studied by resonance Raman spectroscopy. The purified aa(3) oxidase, which does not contain bound quinol, undergoes a reversible slow conformational change at heme a(3) upon reduction, as indicated by a change in the frequency of its heme formyl stretchi...

For many years, Taq polymerase has served as the stalwart enzyme in the PCR amplification of DNA. However, a major limitation of Taq is its inability to amplify damaged DNA, thereby restricting its usefulness in forensic applications. In contrast, Y-family DNA polymerases, such as Dpo4 from Sulfolobus solfataricus, can traverse a wide variety of DNA lesions. Here, we report the identification a...

The study and development of new sustainable mining methods to exploit low-grade ores secondary metallic resources are essential meet global demand contribute caring for the environment. Copper is one most widely used metals chalcocite main sulfide this metal. Therefore, copper recovery from chalcocite-dominant minerals could have a great impact on industry. In study, we assess at bench scale f...