Phosphotransbutyrylase (phosphate butyryltransferase [EC 2.3.1.19]) from Clostridium acetobutylicum ATCC 824 was purified approximately 200-fold to homogeneity with a yield of 13%. Steps used in the purification procedure were fractional precipitation with (NH4)2SO4, Phenyl Sepharose CL-4B chromatography, DEAE-Sephacel chromatography, high-pressure liquid chromatography with an anion-exchange c...

Volatile fatty acids (VFAs), which can be generated by acidogenesis of organic wastes, are important building blocks for chemicals production, and intermediates in many bioprocesses such as microbial lipids production. Important factors affecting the bioconversion VFAs (acetate, propionate butyrate) Yarrowia lipolytica W29 NCYC 2904 growth accumulation were studied. grew efficiently VFAs-based ...

Abstract Basic sanitation is still lacking in many developing countries, resulting significant environmental health risks. The efficient disposal of food waste with potential resource recovery has recently gained much attention. Food considered to be a favourable substrate for the anaerobic digestion (AD) because it easy biodegradability, rich nutrient availability, and high water content. Wast...

This paper focuses on the study of effect electrolysis activated sludge in a microbial cell (MEC) under anaerobic digestion poultry manure. was conducted using bioreactor design with and without electrodes (conventional condition). Measurements pH, redox potential (ORP), total dissolved solids were carried out, as microscopy during treatment gasometry. There an increase yields CH4 CO2 compared ...

a structured mathematical model of anaerobic conversion of complex organic materials in non-ideally cyclic-batch reactors for biogas production has been developed. the  model is based on multiple-reaction stoichiometry (enzymatic hydrolysis, acidogenesis, acetogenesis and methanogenesis), microbial growth kinetics, conventional material balances in the liquid and gas phases for a cyclic-batch r...

This work shows the increase in hydrolysis and acidogenesis of organic fraction urban solid waste (OFUSW) by action fungal hydrolytic enzymes obtained solid-state fermentation (SSF) with Aspergillus niger C25B28 on citrus wastes (CW) without sterilization or addition nutrients. To quantify waste, three treatments were studied triplicate: OFUSW plus 10 % fresh CW (RC treatment), pretreated SSF (...