A series of mouse-human hybrids was prepared from mouse cells deficient in adenine phosphoribosyltransferase (EC 2.4.2.7) and normal human cells. The hybrids were made in medium containing adenine and alanosine, an antimetabolite known to inhibit de novo adenylic acid biosynthesis. The mouse cells, unable to utilize exogenous adenine, were killed in this medium, but the hybrids proliferated as ...

HNE 4-Hydroxy-2-nonenal 5-HT 5-Hydroxytryptamine ATPase Adenosine triphosphatase ALA Alpha-lipoic acid DNA Deoxyribonucleic acid GABA Gamma-amino butyric acid GPX Glutathione peroxidase GAP43 Growth-associated protein 43 NAD+ Nicotinamide adenine dinucleotide oxidized NADP+ Nicotinamide adenine dinucleotide phosphate oxidized NADHP Nicotinamide adenine dinucleotide phosphate reduced NADH Nicoti...

This work investigates quantum transport in symmetrical and asymmetrical borospherene-based molecular junctions with adenine. Adenine is one of the four nucleobases DNA was selected because its excellent properties. The density functional/non-equilibrium Green's function (DFT-NEGF) mathematical approach utilized, which further used to calculate parameters including I–V curve, transmission spect...

RNase P from Bacillus subtilis cleaves in vitro the adenine riboswitch upstream of pbuE, which codes for an adenine efflux pump. The guanine riboswitch, encoded upstream of xpt-pbuX operon, is not cleaved. The cleavage sites do not occur at any predicted structures that should be recognized by RNase P in the theoretical model of the adenine riboswitch. However, it is possible to draw alternativ...

Spontaneous mutations, and those induced by nitrous acid or ultraviolet light, were studied in the haploid fission yeast Schizosaccharomyces pombe. Reverse mutations conferring ability to grow on a minimal medium lacking adenine were scored in adenine auxotrophs, and in di-auxotrophs having requirements for both adenine and methionine. The apparently lower mutability of the ad-1 mutants in the ...

African trypanosomes, like all obligate parasitic protozoa, cannot synthesize purines de novo and import purines from their hosts to build nucleic acids. The purine salvage pathways of Trypanosoma brucei being redundant, none of the involved enzymes is likely to be essential. Nevertheless they can be of pharmacological interest due to their role in activation of purine nucleobase or nucleoside ...

The structural requirements for the binding of purines to adenine phosphoribosyltransferase from monkey liver were explored by the determination of the K; values for 67 purines and purine analogues. Adenine was bound 40 times better than the most effectively bound analogues. Substitutions on the 6-amino group of adenine decreased binding to varying degrees. The hydroxylaminoand hydrazinopurines...