Phosphoinositidase C activities sensitive to purine and pyrimidine nucleotides have been identified earlier in ampulla from Rana ridibunda semicircular canal. The aim of this study was to characterize the pharmacological properties of other P2 receptors borne by this structure. A microassay was developed to measure the binding of [35S]adenosine 5'- O-(2-thiodiphosphate) ([35S]ADPβS) to a few am...

Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of valinomycin resulted in the synthesis of 0.2 to 0.3 nmol of ATP/mg of membrane pro...

Aims Coronary heart disease is one of the leading causes mortality in modern societies. The associated with lack physical activity and expression some genes involved blood cholesterol transportation. Adenosine triphosphate (ATP)-binding cassette transporters G5/G8) ABCG5 ABCG8) adenosine triphosphate-dependent box protein 5 8, a member ABC transporter family, playing crucial role high-density l...

Cells must amplify external signals to orient and migrate in chemotactic gradient fields. We find that human neutrophils release adenosine triphosphate (ATP) from the leading edge of the cell surface to amplify chemotactic signals and direct cell orientation by feedback through P2Y2 nucleotide receptors. Neutrophils rapidly hydrolyze released ATP to adenosine that then acts via A3-type adenosin...

The association between obesity and osteoarthritis (OA) in joints not subjected to mechanical overload, together with the relationship OA metabolic syndrome, suggests that there are systemic factors related disorders involved phenotype of OA. aim this work is study effects palmitate oleate on cellular metabolism an “ vitro ” model human chondrocytes. TC28a2 chondrocyte cell line was used analyz...

In the skin, adenosine triphosphate (ATP) is released from various types of cells by environmental stimuli via nonlytic mechanisms, cell damage, or acute death. Because ATP a potent inducer skin inflammation, it has to be promptly hydrolyzed for achieve homeostasis. Of note, activates mast (MCs) through P2X7R, leading enhanced inflammation. However, MC involvement in impairment inflammation hyd...

1. A study is made of the effect of adenosine triphosphate (ATP) upon the viscosity of solutions of actomyosin in 0.5 M KCl. 2. The observed effects are discussed in terms of an initial drop of the viscosity (viscosity response) and its subsequent slow reversal (recovery effect). The latter is ascribed to a decrease in the ATP concentration through enzymatic hydrolysis. 3. The recovery effect i...

This article reports on a study on fluorescence adenosine triphosphate (ATP) detection by InP/ZnS quantum dots (QDs). We present a spectroscopic analysis displaying the effect of enzymatic reactions of glucose oxidase (GOX) and hexokinase (HEX) on the InP/ZnS quantum dots at physiological pH. The InP/ZnS quantum dots act as glucose sensors in the presence of GOX, Glu and ATP, and their luminesc...

Recently (J Pharmacol Exp Ther 261:580, 1992), we have shown that K562 leukemia cells express a calcium-signaling purinoceptor with characteristics of the P2T receptor subtype for adenosine diphosphate (ADP) previously found only in platelets. Because these results suggested that the P2T receptor may be an early marker for megakaryocytic differentiation, we studied whether this calcium-signalin...

Abstract Birds and mammals produce most adenosine triphosphate (ATP) through mitochondrial oxidative phosphorylation, but when oxygen is not present in sufficient levels, ATP can be produced anaerobic glycolysis. Pyruvate kinase (PK) catalyzes the final step of glycolysis by converting phosphoenolpyruvate diphosphate (ADP) into pyruvate ATP. Lactate dehydrogenase (LDH) important for catalyzing ...