We recently demonstrated that Interferon Regulatory Factor 6 (IRF6) is a transcription factor essential for keratinocyte adhesion. Specifically, it required the recycling and delivery of E-cadherin to plasma membrane. Interestingly, total levels were not altered in absence IRF6, under culture conditions used test adhesion behavior, IRF6 was detected nucleus. protein includes DNA binding domain ...

A change regarding the extent of adhesion - hereafter referred to as adhesion plasticity - between adhesive and less-adhesive states of mammalian cells is important for their behavior. To investigate adhesion plasticity, we have selected a stable isogenic subpopulation of human MDA-MB-468 breast carcinoma cells growing in suspension. These suspension cells are unable to re-adhere to various mat...

Dominant missense mutations in AQP5, encoding the water channel protein Aquaporin 5, underlie diffuse non-epidermolytic palmoplantar keratoderma (NEPPK), a rare skin condition characterized by thickening of epidermis palms and soles. Prior to this, AQP5 was only known be expressed sweat gland cells role this remains unclear. To study skin, we have two patient-derived keratinocyte cell lines exp...

Osteopontin also known as bone sialoprotein (BSP). Osteopontin role as a linking protein. it is an extracellular structural protein and therefore an organic component of bone. Osteoprontin (OPN) binds to several integrin receptors containing α4β1, α9β1, and α9β4 expressed by leukocytes. These receptors have been well-stable to function in cell adhesion, migratio...

Transforming growth factor beta (TGFβ1, β2, and β3) are 25 kDa disulfide-linked homodimers that regulate many aspects of cellular functions, consist of proliferation, differentiation, adhesion and extracellular matrix formation. TGFβs mediate their biological activities by binding of growth factor ligand to two related, functionally distinct, single-pass transmembrane receptor kinases, known as...

The sulfur-binding protein of Thiobacillus ferrooxidans ATCC 23270 was investigated. The protein composition of the bacterium's cell surface changed according to the culture substrate. Sulfur-grown cells showed greater adhesion to sulfur than iron-grown cells. The sulfur-grown cells synthesized a 40-kDa surface protein which was not synthesized by iron-grown cells. The 40-kDa protein had thiol ...

osteopontin also known as bone sialoprotein (bsp). osteopontin role as a linking protein. it is an extracellular structural protein and therefore an organic component of bone. osteoprontin (opn) binds to several integrin receptors containing α4β1, α9β1, and α9β4 expressed by leukocytes. these receptors have been well-stable to function in cell adhesion, migration, and survival in these cells. i...

Proteins are important in bacterial adhesion, but interactions at molecular-scales between proteins and specific functional groups are not well understood. The adhesion forces between four proteins [bovine serum albumin (BSA), protein A, lysozyme, and poly-d-lysine] and COOH, NH2 and OH-functionalized (latex) colloids were examined using colloid probe atomic force microscopy (AFM) as the functi...

Both proteins and polysaccharides are biopolymers present on a bacterial surface that can simultaneously affect bacterial adhesion. To better understand how the combined presence of proteins and polysaccharides might influence bacterial attachment, adhesion forces were examined using atomic force microscopy (AFM) between colloids (COOH- or protein-coated) and polymer-coated surfaces (BSA, lysoz...