Crystal structures of membrane proteins are highly desired for their use in the mechanistic understanding functions and designing new drugs. However, obtaining protein is difficult. One way to overcome this challenge with fusion methods, which have been successfully used determine many proteins, including receptors, enzymes adhesion molecules. Existing strategies can be categorized into N or C ...

Cell adhesion to the extracellular matrix is generally mediated by integrin receptors, which bind to intracellular adhesion proteins that form multi-molecular scaffolding and signalling complexes. The networks of proteins, and their interactions, are dynamic, mechanosensitive and extremely complex. Recent efforts to characterise adhesions using a variety of technologies, including imaging, prot...

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin 5 chain. In addition to red blood cells, Lu/ BCAM proteins are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to the endothelium could potentially contribute to the vaso-oc...

INTRODUCTION Acinetobacter baumannii is involved in various infectious diseases ranging from nosocomial community-acquired infections to those acquired following war or natural disasters. The treatment has become exceedingly difficult partly because the bacterium can form biofilms. Therefore, it is imperative to elucidate mechanisms of the biofilm formation that may be exploited to develop ther...

Calcium phosphate cements (CPCs) are attractive synthetic bone grafts as they possess osteoconductive and osteoinductive properties. Their biomimetic synthesis grants them an intrinsic nano- microporosity that resembles natural is paramount for biological processes such protein adhesion, which can later enhance cell adhesion. However, a main limitation of CPCs the lack macroporosity, crucial to...

Within the biomaterials proposed for tissue regeneration, synthetic 3D hydrogels that mimic soft tissues possess great potential regenerative medicine but their poor vascularization rate is usually incompatible with long-term cell survival. Fabrication of promote and/or accelerate remains nowadays a challenge. In present work, tubular geometries ranging from 28 to 680 μm in diameter, correspond...

Both proteins and polysaccharides are biopolymers present on a bacterial surface that can simultaneously affect bacterial adhesion. To better understand how the combined presence of proteins and polysaccharides might influence bacterial attachment, adhesion forces were examined using atomic force microscopy (AFM) between colloids (COOH- or protein-coated) and polymer-coated surfaces (BSA, lysoz...

Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the alpha(2), alpha(5) a...

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. In addition to red blood cells, Lu/BCAM proteins are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to the endothelium could potentially contribute to the v...