DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

We show that nano-mechanical interaction using atomic force microscopy (AFM) can be used to map out mode-patterns of an optical micro-resonator with high spatial accuracy. Furthermore we demonstrate how the Q-factor and center wavelength of such resonances can be sensitively modified by both horizontal and vertical displacement of an AFM tip consisting of either Si(3)N(4) or Si material. With a...

Biological processes such as endocytosis/exocytosis, interactions between cells and the dynamic reorganization of the cytosqueleton are between the topics extensively studied using the Atomic Force Microscopy (AFM) technique. The topographical structure of the cell membrane itself is complex, rich in natural corrugations and ridges. These surface characteristics obscure the identification of ex...

Thiol self-assembled monolayers (SAMs) are widely used in many nano- and bio-technology applications. We report a new approach to create and characterize a thiol SAMs micropattern with alternating charges on a flat gold-coated substrate using atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM). We produced SAMs-patterns made of alternating positively charged, negatively charg...

A well-defined test structure of organic static-induction transistor (SIT) having regularly sized nano-apertures in the gate electrode has been fabricated by colloidal lithography using 130-nm-diameter polystyrene spheres as shadow masks during vacuum deposition. Transistor characteristics of individual nano-apertures, namely ‘nano-SIT,’ have been measured using a conductive atomic-force-micros...

A new experimental method is introduced in order to characterize the mechanical properties of metallic nanowires. An accurate mechanical characterization of nanowires requires simultaneous imaging using scanning electron microscope (SEM) and mechanical testing with an atomic force microscope (AFM). In this study, an AFM is located inside an SEM chamber in order to establish the visibility of th...

Titanium thin layers with the same deposition conditions were deposited on glass substrates at room temperature. Different annealing temperatures namely, 400 K, 500 K and 600 K with uniform 9 cm /sec oxygen 3 flow were used to produce titanium oxide layers. The nano-structure of layers were studied using AFM and XRD methods. Optical Reflectance were studied by spectrophotometer method. Correlat...

This paper proposes two different nanomolding methods to fabricate synthetic gecko foot-hair nanostructures. The first method uses Atomic Force Microscope (AFM) probe indented flat wax surface and the second one uses a nano-pore membrane as a template. These templates are molded with silicone rubber, polyamide and polyester type of polymers under vacuum and the template is peeled off or etched ...