The Box-Wilson central composite design (CCD) based on response surface methodology (RSM) was used for ethanol fermentation using very high gravity (VHG) finger millet hydrolysate. Optimized process variables were namely, concentrations of yeast extract, magnesium sulphate and pH of the medium. High gravity mashes (>300 g dissolved solids per liter) were prepared by a thermo-stable α-amylase, f...

Toll-like receptors (TLRs) play a fundamental role in the immune system by detecting pathogen associated molecular patterns (PAMPs) to sense host infection. Ethanol at doses relevant for humans inhibits the pathogen induced cytokine response mediated through TLRs. The current study was designed to investigate the mechanisms of this effect by determining whether ethanol inhibits TLR3 and TLR4 me...

The inactivation of virus-contaminated nonporous inanimate surfaces was investigated using adenovirus type 8, a common cause of epidemic keratoconjunctivitis. A 10-microl inoculum of adenovirus was placed onto each stainless steel disk (1-cm diameter), and the inoculum was allowed to air dry for 40 min. Twenty-one different germicides (including disinfectants and antiseptics) were selected for ...

Alterations in N-methyl-d-aspartate receptor (NMDAR) protein levels or subcellular localization in brain after chronic ethanol exposure may contribute to withdrawal-associated seizures and neurotoxicity. We have investigated synaptic localization of NMDARs in cultured hippocampal pyramidal neurons after prolonged (7 days) exposure to, and acute withdrawal from, 80 mM ethanol using fluorescence ...

This study evaluated the influence of the surface treatments of fiberglass posts on bond strength to root dentin using push-out test. Forty bovine incisor roots were endodontically treated. The surface of the fiberglass posts (Exacto #2, Angelus) were treated using 4 different protocols (n=10): Control - 70% ethanol for 1 min; 37% phosphoric acid for 1 min; 10% hydrofluoric acid for 1 min; and ...

Fixation in 10% neutral buffered formalin prior to transfer to 70% ethanol for one week has been shown to adequately preserve immunorecognition of PCNA, cytokeratins AE1/AE3 and EGFr. This study investigated whether 12 hrs fixation in 10% NBF plus transfer to 70% ethanol for 4 weeks would similarly preserve immunorecognition to an extent where antigen retrieval (AR) used to reverse the masking ...

abstract alstroemeria is one of the most important cut flowers in the world which is multiplicated by rhizome explants in in vitro conditions . the major problem in using rhizome explants is fungal and bacterial contamination which inhibit the preparation of strill explants. therefor in this experiment, the effect of disinfecting compounds like sodium hypochlorite, mercury chloride, nano silver...

Article history: Received: 17 May 2013 Accepted: 19 June 2013 Available online: 28 Sep 2013 ZJRMS 2015 Feb; 17(2): 22-26 Background: Leptin is one of the possible mediators of ethanol intake. On the other hand, the concentration of total plasma homocysteine (Hcy) is a well-established indicator for the risk of cardiovascular disease and seems to be related to ethanol consumption. So, the aim of...

The substituted pyrrole monomer, N–(2–cyanoethyl)pyrrole, was electropolymerised in a 70% water and 30% ethanol solution with ClO4– H2PO4– as the dopant species to give nanowires at short electropolymerisation times microwires longer deposition periods. On adding toluene solution, hollow microtubes were formed. This attributed adsorption of droplets electrode surface which served separate dopan...

An ovigerous female of Argulus matuii Sikama, 1938 was collected from the body surface a yellowfin seabream Acanthopagrus latus (Houttuyn, 1782) in cove facing Bungo Channel off Ehime Prefecture, western Japan. This represents new host record for A. matuii, and is herein described. female, which fixed preserved 70% ethanol, showed prominent coloration, i.e., six streaks yellow fringed with dark...