34 nm gold nanoparticles with good stability were synthesized and characterized and their effect (as a function of concentration) on the proliferation of keratinocytes was evaluated by means of MTT and nucleolar organizer region (AgNOR) count (silver staining). The cell morphology was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results demonstr...

In malignant lymphomas, cell kinetics has shown to be related with histologic type as well as with the clinical behaviour. The aim of our study was to investigate the relevance of cell proliferation parameters on overall survival in non-Hodgkin's lymphomas as well as their relationship with prognostic factors such as International Prognostic Index (IPI). We performed DNA-flow-cytometry (S-phase...

The aim of the present study was to investigate if the evaluation of cell proliferation of the well differentiated squamous cell carcinoma (WDSCC) and infundibular keratinizing acanthoma (IKA) could be useful in the differential diagnosis between these two tumours. Eighteen IKAs and ten WDSCCs were selected for this study. Two different methods were used to assess the activity of cell prolifera...

The aim of this study was to define the relation between silver stained nucleolar organizer regions (AgNORs) count and histological grade, and clinical stage in needle biopsy specimens of 65 prostatic adenocarcinomas. Histological grade was determined according to the modified Gleason system. AgNORs were evaluated by the counting method on routine histological sections stained with silver. Ther...

A cytogenetic study was conducted on five species of the genus Pimelodus collected from various regions in Brazil: Pimelodus heraldoi, Pimelodus sp, Pimelodus argenteus, P. maculatus and P. mysteriosus. All the species had 2n=56 chromosomes. AgNOR was found in one pair of subtelocentric (ST) chromosomes with staining at the terminal position in all the five species. However, Pimelodus sp, P. he...

AIM To determine the potential value of interphase cytogenetic and argyrophilic nucleolar organiser region (AgNOR) analyses in the diagnosis and classification of hydatidiform moles. METHODS Serial tissue sections from 37 hydatidiform moles, histologically classified as 11 complete and 15 partial, and from 11 hydropic abortuses were examined by in situ hybridisation using digoxigenin labelled...

AIM To investigate the prognostic value of argyrophylic nucleolar organiser regions (AgNORs) in multiple myeloma. METHODS Bone marrow aspirates from 55 newly diagnosed patients with multiple myeloma were stained with the one step AgNO3 technique. The mean number of AgNORs in each plasma cell nucleus (AgNOR count) was tested for a possible correlation with other clinical and laboratory variabl...

STUDY OBJECTIVES To evaluate the usefulness of argyrophilic nucleolar organizer region (AgNOR) counting and flow cytometric DNA analysis in the differential diagnosis of thymoma and thymic carcinoma, as well as in the differences among various stages and histologic subtypes of these tumors. DESIGN AND INTERVENTIONS Paraffin-embedded blocks of 64 thymic epithelial tumors (20 noninvasive thymom...

BACKGROUND Prediction of radiation response before the completion of the radiotherapy schedule is challenging. Information about radiation response could help oncologist to choose the appropriate combination and sequence of therapies in the multidisciplinary management of cancer. METHODS The study involved 26 patients with squamous cell cancers of the head and neck region who received radioth...