PURPOSE There is a need to develop predictive tests that would allow identifying cancer patients with a high risk of developing side effects to radiotherapy. We compared the predictive value of three functional assays: the G(0) aberration assay, the G(2) aberration assay and the alkaline comet assay in lymphocytes of breast cancer and gynaecological cancer patients. MATERIAL AND METHODS Perip...

background: alpinia galanga l. belonging to the family zingiberaceae is widely grown in the state of kerala, india. they are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. the efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic dna was studied. materials and methods: the bioflavonoids, galangin and k...

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances complex mixtures that are released into the environment can lead to contamination of surface subsurface waters. Such activities have endangered existence ecosystems their inhabitants. Changes in genome caused by genotoxic agents led mutations pose a burden populations fish species. In present study, ...

background traditional medicines are among the oldest medicines and their extensive use in the recent years reflects the public’s interest in alternatives to conventional medicine. objectives the aim of this study was to investigate the genotoxicity of dillsun herbal medicine in dna damage of rat hepatocytes compared to sodium dichromate using a comet assay technique. materials and methods male...

Objective: To investigate the radioprotecting ability of the extract of the medicinal plant Holarrhena antidysentrica, especially to protect DNA from gammaradiation induced damages. Materials and Methods: Hydo-alcoholic extract of dried roots of Holarrhena antidysentrica (HAE) was prepared, freeze dried and stored at 4C. The DNA of the plasmid pBR322 was exposed to gamma-radiation in presence a...

The comet assay is actually one of the most widespread tool to evaluate DNA damage. Its sensibility to low dose radiation has been tested in vitro, both there is a limited data showing its application and sensibility in chronic exposure situation, where the assay seem to be more useful. The influence of the internal contamination caused by the Chernobyl accident on the level of DNA damage was e...

use of different pesticides in the agriculture sector, in order to boost crop yield within a short time period and low labor, has been tremendously increased since the last decade. pesticide use has elevated crop yield but has produced a number of pronounced problems regarding environmental and health safety. the continuously deteriorating toxicological effects of these pesticides are not only ...

The alkaline single-cell gel electrophoresis (SCGE) assay, also called the comet assay, is a rapid and simple method for the detection of DNA damage in individual cells. The objective of this study was to establish if the alkaline SCGE assay in whole blood cells gives similar results as the same method in isolated lymphocytes, because whole blood cells are simpler and more economical to use, sp...

AIM This study aims at evaluating the chromosomal abnormalities and deoxyribonucleic acid (DNA) damage in cases with primary amenorrhea by karyotyping and comet assay. STUDY DESIGN A total of 30 cases of primary amenorrhea were recruited. Secondary sexual characters were assessed by Tanner staging. Chromosomal analysis was performed by conventional phytohemagglutinin stimulated lymphocyte cel...

INTRODUCTION Drug delivery systems could induce cellular toxicity as side effect of nanomaterials. The mechanism of toxicity usually involves DNA damage. The comet assay or single cell gel electrophoresis (SCGE) is a sensitive method for detecting strand damages in the DNA of a cell with applications in genotoxicity testing and molecular epidemiology as well as fundamental research in DNA damag...