The present study was designed to examine the effect of Pseudomonas aeruginosa alkaline protease and elastase on human polymorphonuclear leukocyte chemiluminescence. Both a luminol-enhanced and a nonenhanced chemiluminescence system using opsonized zymosan were utilized. It was found that alkaline protease and elastase at concentrations of 25 micrograms/ml strongly inhibited luminol-enhanced my...

PURPOSE To establish if active pseudomonal proteases are present in vivo during corneal infection with Pseudomonas aeruginosa and to determine if the mouse strains used in these and previous studies have the ability to mount a nonocular antibody response to the purified proteases because antibodies to the bacterial proteases were not detected previously during in vivo ocular infection. METHOD...

An alkali tolerant Bacillus strain having extracellular serine alkaline protease activity was newly isolated from compost and identified as Bacillus clausii GMBE 22. An alkaline protease (AP22) was 4.66-fold purified in 51.5% yield from Bacillus clausii GMBE 22 by ethanol precipitation and DEAE-cellulose anion exchange chromatography. The purified enzyme was identified as serine protease by LC-...

Alkaline proteases are the most important groups of commercial enzymes, which have been broadly used in industrial processes. In this study, Bacillus sp. PTCC 1538 was selected as a biological agent to produce alkaline protease. Enzyme production under submerge fermentation using waste effluent investigated. Since costs raw material plays an role cost enzyme production, corn steep liquor (CSL) ...

Isolation and optimization of alkaline protease producing Bacteria from undisturbed soil NE-region India falling under Indo-Burma biodiversity hotspotsOnkar Nath Tiwari, Thiyam Bidyababy Devi, Kangjam Sarabati Gunapati Oinam, Thingujam Indrama, Keithellakpam Ojit, Oinam Avijeet, Lakreiphy Ningshen

In the present study, forty-five bacterial isolates were obtained from previously unstudied soil samples in Bal?kl?göl, ?anl?urfa. Based on their enzim production capacities, six designated as BGL-22, BGL-26, BGL-27, BGL-37, BGL-38 and BGL-39 selected for further studies. Conventional molecular identification results showed that bacteria belonged to Bacillus genus. Among these strains, highest ...

Enzyme solutiens of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles otsupercritical carbon dioxide (SC CO,) fed from a cylindrical filter nozzle. The microbllbbles of SC C02 could increase the CO, concentration in the sample solution from O.4 to O.92molfl at 25 MPa and 350C, and hence could improye the eMciency of inactiyation by about 3 times compared ...